Human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction

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dc.contributor.authorJ Ahn-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorJ Lee-
dc.contributor.authorM G Kim-
dc.date.accessioned2017-04-19T10:03:44Z-
dc.date.available2017-04-19T10:03:44Z-
dc.date.issued2015-
dc.identifier.issn09565663-
dc.identifier.uri10.1016/j.bios.2015.03.073ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12583-
dc.description.abstractThe aim of the study was to develop a simple and rapid immunoassay using fluorescent microbeads and enzyme-substrate reactions to measure alpha-fetal protein (AFP) concentrations. We demonstrated the functionality of the fluorescent immunosensor using antibody-conjugated fluorescent latex beads (AB-FLBs) and horseradish peroxidase (HRP) to catalyze a reaction, where the products would precipitate and suppress the fluorescence of AB-FLBs. First, the AB-FLBs were incubated with antigen, biotinylated antibodies (bABs), and streptavidin-HRP (SAv-HRP) to form a sandwich-type immunoreaction. The mixture was then filtered through a membrane to concentrate the beads on a small area. After washing to remove unbound bABs and SAv-HRP, a chromogenic HRP substrate and H2O2 were added to form precipitates on the FLB surface. The suppression of the fluorescence was measured with a fluorescent image analyzer system. Under optimized conditions, AFP could be measured at concentrations as low as 1 pg mL-1 with a dynamic range up to 100 ng mL-1-
dc.publisherElsevier-
dc.titleHuman alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction-
dc.title.alternativeHuman alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage120-
dc.citation.startPage115-
dc.citation.volume71-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.alternativeName안준형-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName이재종-
dc.contributor.alternativeName김민곤-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 71, pp. 115-120-
dc.identifier.doi10.1016/j.bios.2015.03.073-
dc.subject.keywordAlpha-fetal protein (AFP)-
dc.subject.keywordEnzyme-catalyzed precipitation-
dc.subject.keywordFluorescence suppression-
dc.subject.keywordFluorescent immunoassay-
dc.subject.localAlpha-fetal protein (AFP)-
dc.subject.localEnzyme-catalyzed precipitation-
dc.subject.localFluorescence suppression-
dc.subject.localFluorescent immunoassay-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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