DC Field | Value | Language |
---|---|---|
dc.contributor.author | J Ahn | - |
dc.contributor.author | Yong Beom Shin | - |
dc.contributor.author | J Lee | - |
dc.contributor.author | M G Kim | - |
dc.date.accessioned | 2017-04-19T10:03:44Z | - |
dc.date.available | 2017-04-19T10:03:44Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | 10.1016/j.bios.2015.03.073 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12583 | - |
dc.description.abstract | The aim of the study was to develop a simple and rapid immunoassay using fluorescent microbeads and enzyme-substrate reactions to measure alpha-fetal protein (AFP) concentrations. We demonstrated the functionality of the fluorescent immunosensor using antibody-conjugated fluorescent latex beads (AB-FLBs) and horseradish peroxidase (HRP) to catalyze a reaction, where the products would precipitate and suppress the fluorescence of AB-FLBs. First, the AB-FLBs were incubated with antigen, biotinylated antibodies (bABs), and streptavidin-HRP (SAv-HRP) to form a sandwich-type immunoreaction. The mixture was then filtered through a membrane to concentrate the beads on a small area. After washing to remove unbound bABs and SAv-HRP, a chromogenic HRP substrate and H2O2 were added to form precipitates on the FLB surface. The suppression of the fluorescence was measured with a fluorescent image analyzer system. Under optimized conditions, AFP could be measured at concentrations as low as 1 pg mL-1 with a dynamic range up to 100 ng mL-1 | - |
dc.publisher | Elsevier | - |
dc.title | Human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction | - |
dc.title.alternative | Human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction | - |
dc.type | Article | - |
dc.citation.title | Biosensors & Bioelectronics | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 120 | - |
dc.citation.startPage | 115 | - |
dc.citation.volume | 71 | - |
dc.contributor.affiliatedAuthor | Yong Beom Shin | - |
dc.contributor.alternativeName | 안준형 | - |
dc.contributor.alternativeName | 신용범 | - |
dc.contributor.alternativeName | 이재종 | - |
dc.contributor.alternativeName | 김민곤 | - |
dc.identifier.bibliographicCitation | Biosensors & Bioelectronics, vol. 71, pp. 115-120 | - |
dc.identifier.doi | 10.1016/j.bios.2015.03.073 | - |
dc.subject.keyword | Alpha-fetal protein (AFP) | - |
dc.subject.keyword | Enzyme-catalyzed precipitation | - |
dc.subject.keyword | Fluorescence suppression | - |
dc.subject.keyword | Fluorescent immunoassay | - |
dc.subject.local | Alpha-fetal protein (AFP) | - |
dc.subject.local | Enzyme-catalyzed precipitation | - |
dc.subject.local | Enzyme-Catalyzed precipitation | - |
dc.subject.local | Fluorescence suppression | - |
dc.subject.local | Fluorescent immunoassay | - |
dc.description.journalClass | Y | - |
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