A high throughput soybean gene identification system developed using Soybean Yellow Common Mosaic Virus (SYCMV)

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dc.contributor.authorE Y Seo-
dc.contributor.authorS Cho-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorT Gotoh-
dc.contributor.authorH G Kim-
dc.contributor.authorL L Domier-
dc.contributor.authorS Lim-
dc.contributor.authorK H Kim-
dc.contributor.authorJ K Moon-
dc.contributor.authorJ Hammond-
dc.contributor.authorH S Lim-
dc.contributor.authorK H Song-
dc.date.accessioned2017-04-19T10:03:45Z-
dc.date.available2017-04-19T10:03:45Z-
dc.date.issued2015-
dc.identifier.citationJournal of the Faculty of Agriculture, Kyushu University ,60,1,127,138ko
dc.identifier.issn0023-6152-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12585-
dc.description.abstractSoybean yellow common mosaic virus (SYCMV) was recently reported from Korea, and a subsequent survey of soybean fields found that SYCMV, Soybean yellow mottle mosaic virus (SYMMV), and Soybean mosaic virus (SMV) infections were widespread. SYCMV has recently been developed into a Virus Inducing Gene Silencing (VIGS) vector for use as a reverse genetics tool for soybean, and here we report a modified SYCMV VIGS vector containing a new restriction enzyme site in the 3′ non-coding region into which we inserted the Gateway system. Ultrasonically generated c.300 bp random fragments of Glycine max cDNA were inserted into the SYCMV VIGS vector, and individual colonies containing G. max cDNA were inoculated to cultivar Williams 82. We monitored the phenotype of inoculated soybean, and selected obvious visible phenotypes caused by SYCMV-induced gene silencing which could enable annotation of gene function of unknown gene fragments inserted into SYCMV. Here, we describe development of a high-throughput SYCMV VIGS vector for gene function identification in soybean.-
dc.publisherKyushu Univ, Faculty Agricultural Publications-
dc.titleA high throughput soybean gene identification system developed using Soybean Yellow Common Mosaic Virus (SYCMV)-
dc.title.alternativeA high throughput soybean gene identification system developed using Soybean Yellow Common Mosaic Virus (SYCMV)-
dc.typeArticle-
dc.citation.titleJournal of Faculty of Agriculture Kyushu University-
dc.citation.number1-
dc.citation.endPage138-
dc.citation.startPage127-
dc.citation.volume60-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeNameGotoh-
dc.contributor.alternativeName-
dc.contributor.alternativeNameDomier-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeNameHammond-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.identifier.bibliographicCitationJournal of Faculty of Agriculture Kyushu University, vol. 60, no. 1, pp. 127-138-
dc.subject.keywordGateway cloning system-
dc.subject.keywordReverse genetics-
dc.subject.keywordSoybean cDNA library-
dc.subject.keywordSoybean yellow common mosaic virus-
dc.subject.keywordVirus induced gene silencing-
dc.subject.localGateway cloning system-
dc.subject.localReverse Genetics-
dc.subject.localReverse genetics-
dc.subject.localSoybean cDNA library-
dc.subject.localSoybean yellow common mosaic virus-
dc.subject.localSoybean yellow common mosaic virus (SYCMV)-
dc.subject.localVirus induced gene silencing-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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