Differences in the gene expression profiles of slow- and fast-forming preinduced pluripotent stem cell colonies

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dc.contributor.authorSujin Kwon-
dc.contributor.authorJung Sun Park-
dc.contributor.authorByungkuk Min-
dc.contributor.authorYong-Kook Kang-
dc.date.accessioned2017-04-19T10:05:19Z-
dc.date.available2017-04-19T10:05:19Z-
dc.date.issued2015-
dc.identifier.issn1687-966X-
dc.identifier.uri10.1155/2015/195928ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12617-
dc.description.abstractInduced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of stochastic events. In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and fast-forming NGFP-iPSCs, have equivalent levels of pluripotency. Multiplex reverse-transcriptase PCR generated gene expression profiles (GEPs) of 13 pluripotency genes in single initially formed-iPSC (if-iPSC) colonies of NGFP and 4F2A group. Assessment of GEP difference using a weighted root mean square deviation (wRMSD) indicates that 4F2A if-iPSCs are more closely related to mESCs than NGFP if-iPSCs. Consistently, Nanog and Sox2 genes were more frequently derepressed in 4F2A if-iPSC group. We further examined 20 genes that are implicated in reprogramming. They were, overall, more highly expressed in NGFP if-iPSCs, differing from the pluripotency genes being more expressed in 4F2A if-iPSCs. wRMSD analysis for these reprogramming-related genes confirmed that the 4F2A if-iPSC colonies were less deviated from mESCs than the NGFP if-iPSC colonies. Our findings suggest that more important in attaining a better reprogramming is the mode of action by the given reprogramming factors, rather than the total activity of them exerting to the cells, as the thin-but-long-lasting mode of action in 4F2A if-iPSCs is shown to be more effective than its full-but-short-lasting mode in NGFP if-iPSCs.-
dc.publisherHindawi Ltd-
dc.titleDifferences in the gene expression profiles of slow- and fast-forming preinduced pluripotent stem cell colonies-
dc.title.alternativeDifferences in the gene expression profiles of slow- and fast-forming preinduced pluripotent stem cell colonies-
dc.typeArticle-
dc.citation.titleStem Cells International-
dc.citation.number0-
dc.citation.endPage195928-
dc.citation.startPage195928-
dc.citation.volume2015-
dc.contributor.affiliatedAuthorSujin Kwon-
dc.contributor.affiliatedAuthorJung Sun Park-
dc.contributor.affiliatedAuthorByungkuk Min-
dc.contributor.affiliatedAuthorYong-Kook Kang-
dc.contributor.alternativeName권수진-
dc.contributor.alternativeName박정선-
dc.contributor.alternativeName민병국-
dc.contributor.alternativeName강용국-
dc.identifier.bibliographicCitationStem Cells International, vol. 2015, pp. 195928-195928-
dc.identifier.doi10.1155/2015/195928-
dc.description.journalClassY-
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