An enhanced ascorbate peroxidase 2/antibodybinding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier
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- Title
- An enhanced ascorbate peroxidase 2/antibodybinding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier
- Author(s)
- J Lee; E K Song; Y Bae; J Min; H W Rhee; T J Park; Moonil Kim; S Kang
- Bibliographic Citation
- Chemical Communications, vol. 51, no. 54, pp. 10945-10948
- Publication Year
- 2015
- Abstract
- A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
- ISSN
- 1359-7345
- Publisher
- Royal Soc Chem
- Type
- Article
- Appears in Collections:
- Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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