An enhanced ascorbate peroxidase 2/antibodybinding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier

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Title
An enhanced ascorbate peroxidase 2/antibodybinding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier
Author(s)
J Lee; E K Song; Y Bae; J Min; H W Rhee; T J Park; Moonil Kim; S Kang
Bibliographic Citation
Chemical Communications, vol. 51, no. 54, pp. 10945-10948
Publication Year
2015
Abstract
A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
ISSN
1359-7345
Publisher
Royal Soc Chem
DOI
http://dx.doi.org/10.1039/c5cc02409a
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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