Isolation of foreign material-free endothelial progenitor cells using CD31 aptamer and therapeutic application for ischemic injury

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dc.contributor.authorJ W Yoon-
dc.contributor.authorI H Jang-
dc.contributor.authorS C Heo-
dc.contributor.authorY W Kwon-
dc.contributor.authorE J Choi-
dc.contributor.authorKwang-Hee Bae-
dc.contributor.authorD S Suh-
dc.contributor.authorS C Kim-
dc.contributor.authorS Han-
dc.contributor.authorS Haam-
dc.contributor.authorJ Jung-
dc.contributor.authorK Kim-
dc.contributor.authorS H Ryu-
dc.contributor.authorJ H Kim-
dc.date.accessioned2017-04-19T10:08:26Z-
dc.date.available2017-04-19T10:08:26Z-
dc.date.issued2015-
dc.identifier.issn19326203-
dc.identifier.uri10.1371/journal.pone.0131785ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12707-
dc.description.abstractEndothelial progenitor cells (EPCs) can be isolated from human bone marrow or peripheral blood and reportedly contribute to neovascularization. Aptamers are 40-120-mer nucleotides that bind to a specific target molecule, as antibodies do. To utilize apatmers for isolation of EPCs, in the present study, we successfully generated aptamers that recognize human CD31, an endothelial cell marker. CD31 aptamers bound to human umbilical cord blood-derived EPCs and showed specific interaction with human CD31, but not with mouse CD31. However, CD31 aptamers showed non-specific interaction with CD31-negative 293FT cells and addition of polyanionic competitor dextran sulfate eliminated non-specific interaction without affecting cell viability. From the mixture of EPCs and 293FT cells, CD31 aptamers successfully isolated EPCs with 97.6% purity and 94.2% yield, comparable to those from antibody isolation. In addition, isolated EPCs were decoupled from CD31 aptamers with a brief treatment of high concentration dextran sulfate. EPCs isolated with CD31 aptamers and subsequently decoupled from CD31 aptamers were functional and enhanced the restoration of blood flow when transplanted into a murine hindlimb ischemia model. In this study, we demonstrated isolation of foreign material-free EPCs, which can be utilized as a universal protocol in preparation of cells for therapeutic transplantation.-
dc.publisherPublic Library of Science-
dc.titleIsolation of foreign material-free endothelial progenitor cells using CD31 aptamer and therapeutic application for ischemic injury-
dc.title.alternativeIsolation of foreign material-free endothelial progenitor cells using CD31 aptamer and therapeutic application for ischemic injury-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number7-
dc.citation.endPagee0131785-
dc.citation.startPagee0131785-
dc.citation.volume10-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.alternativeName윤정원-
dc.contributor.alternativeName장일호-
dc.contributor.alternativeName허순철-
dc.contributor.alternativeName권양우-
dc.contributor.alternativeName최은정-
dc.contributor.alternativeName배광희-
dc.contributor.alternativeName서동수-
dc.contributor.alternativeName김승철-
dc.contributor.alternativeName한승민-
dc.contributor.alternativeName함승주-
dc.contributor.alternativeName정종하-
dc.contributor.alternativeName김기석-
dc.contributor.alternativeName류성호-
dc.contributor.alternativeName김재호-
dc.identifier.bibliographicCitationPLoS One, vol. 10, no. 7, pp. e0131785-e0131785-
dc.identifier.doi10.1371/journal.pone.0131785-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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