Rapid generation of secondary fibroblasts through teratoma formation

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Title
Rapid generation of secondary fibroblasts through teratoma formation
Author(s)
Sang Mi ChoJung Sun Park; Byongkuk Min; SuJin Kwon; Yong-Kook Kang
Bibliographic Citation
Biotechniques, vol. 59, no. 1, pp. 34-41
Publication Year
2015
Abstract
The use of secondary or reprogrammable cells in the production of induced pluripotent stem cells (iPSCs) circumvents random infection by various viral particles and random, uncontrollable integrations of the viral genomes into different genomic loci. We have developed a convenient method for repeatedly producing genetically identical secondary fibroblasts via teratoma formation using pre-existing IPSCS. The iPSCs used in this study carried doxycycline (Dox)-inducible transgenes for four transcription factors in their genome. Teratoma-derived primary cells (TOFs) were obtained in a huge amount during the culture of teratomas and showed good ability to form iPSCs similar to that of regular secondary fibroblasts. Immunohisto chemistry analysis demonstrated the potential of TOF-derived iPSCs to differentiate into all three germ layers. The gene expression profiles of these TOFs and their iPSCs closely mimicked those of regular embryonic fibroblasts and embryonic stem cells/iPSCs, respectively. The possibility that the iPSCs were derived from a small part of pluripotent cells lurking in the TOF population was precluded by the observation of doxycycline-dependent and PluriSin (a compound selectively eliminating pluripotent cells)-independent formations of iPSCs. Our results showed that the TOFs retained the capability to mediate cellular reprogramming, similar to that of regular secondary fibroblasts.
Keyword
DoxycyclineInduced pluripotent stem cell (iPSC)Mouse embryonic fibroblast (MEF)ReprogrammingSecondary fibroblastStem cellTeratoma
ISSN
0736-6205
Publisher
Future Sci Ltd
DOI
http://dx.doi.org/10.2144/000114309
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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