In vivo toxicity assessment of angiogenesis and the live distribution of nano-graphene oxide and its PEGylated derivatives using the developing zebrafish embryo = 제브라피쉬 배아 이용 나노그래핀의 생체내 혈관발생 독성 검증 및 생체내 분포

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Title
In vivo toxicity assessment of angiogenesis and the live distribution of nano-graphene oxide and its PEGylated derivatives using the developing zebrafish embryo = 제브라피쉬 배아 이용 나노그래핀의 생체내 혈관발생 독성 검증 및 생체내 분포
Author(s)
Jin Young JeongHyun Ju Cho; Mi Jin Choi; Wang Sik Lee; Bong Hyun Chung; Jeong Soo Lee
Bibliographic Citation
Carbon, vol. 93, pp. 431-440
Publication Year
2015
Abstract
The toxicity of nano-graphene oxide (NGO) on development and angiogenesis was evaluated using zebrafish embryos as in vivo model system. Microinjection of NGO resulted in gross morphological defects in a dose-dependent manner partly due to the induction of apoptosis, whereas coating NGO derivatives with polyethylene glycol (PEG), a biocompatible polymer significantly attenuated its toxicity. NGO also caused abnormal branching and mispatterning of developing trunk blood vessels monitored by endothelial cell-specific fluorescent transgenic zebrafish, presumably via Vascular Endothelial Growth Factor and Notch pathways, key signaling pathways for normal angiogenic process. Interestingly, Alexa568 conjugated, PEG-coated NGO (NGO-A568) still caused angiogenic defects to the similar degree as NGO did, suggesting differential toxic effects of nanomaterials on different developmental processes. Confocal microscopy imaging of NGO- and NGO-A568-injected embryos visualized its live distribution throughout the body including the cranial vasculature. The broader utilization of embryonic zebrafish combined with in vivo, real-time, high-resolution imaging is warranted for assessing the properties of nanomaterials.
ISSN
0008-6223
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.carbon.2015.05.024
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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