Scenedesmus-based treatment of nitrogen and phosphorus from effluent of anaerobic digester and bio-oil production

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Title
Scenedesmus-based treatment of nitrogen and phosphorus from effluent of anaerobic digester and bio-oil production
Author(s)
G Y Kim; Y M Yun; H S Shin; Hee-Sik Kim; J I Han
Bibliographic Citation
Bioresource Technology, vol. 196, pp. 235-240
Publication Year
2015
Abstract
Myoglobin is an early biomarker for acute myocardial infarction. Recently, we isolated the antibody IgG-Myo2-7ds, which exhibits unique rapid reaction kinetics toward human myoglobin antigen. Antibodies with rapid dissociation kinetics are thought to be premature IgG forms that are produced during the early stage of in vivo immunization. In the present study, we identified the epitope region of the IgGMyo2- 7ds antibody to be the C-terminal region of myoglobin, which corresponds to 144-154 aa. The Fab fragment was directly purified by papain cleavage and protein G affinity chromatography and demonstrated kinetics of an association constant of 4.02 × 104 M-1 s-1 and a dissociation constant of 2.28 × 10-2 s-1, which retained the unique reaction kinetics of intact IgG-Myo2-7ds antibodies. Because a rapid dissociation antibody can be utilized for antibody recycling, the results from this study would provide a platform for the development of antibody engineering in potential diagnostic areas such as a continuous monitoring system for heart disease.
Keyword
Acute myocardial infarctionMyoglobinPremature antibodyProtein GRapid reaction kinetics
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2015.07.091
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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