Pseudoseohaeicola caenipelagi gen. nov., sp. Nov., isolated from a tidal flat

Abstract

A Gram-stain-negative, non-motile, aerobic and pleomorphic bacterium, designated BS-W13T, was isolated from a tidal flat on the South Sea, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BS-W13T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0% (w/v) NaCl. Neighbour-joining and maximumparsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain BS-W13T clustered with the type strain of Seohaeicola saemankumensis, showing the highest sequence similarity (95.96 %) to this strain. Strain BS-W13T exhibited 16S rRNA gene sequence similarity values of 95.95, 95.91, 95.72 and 95.68% to the type strains of Sulfitobacter donghicola, Sulfitobacter porphyrae, Sulfitobacter mediterraneus and Roseobacter litoralis, respectively. Strain BS-W13Tcontained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The polar lipid profile of strain BS-W13T, containing phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid as major components, was distinguishable from those of some phylogenetically related taxa. The DNA G+C content of strain BS-W13T was 58.1 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain BS-W13Tconstitutes a novel genus and species within family Rhodobacteraceae of the class Alphaproteobacteria, for which the name Pseudoseohaeicola caenipelagi gen. Nov, sp. nov. is proposed. The type strain is BS-W13T(=KCTC 42349T=CECT 8724T).