Acquisition of the depth profiles and reproducible mass spectra in matrix-assisted laser desorption/ionization of inhomogeneous samples

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dc.contributor.authorS H Ahn-
dc.contributor.authorK M Park-
dc.contributor.authorJeong Hee Moon-
dc.contributor.authorS H Lee-
dc.contributor.authorM S Kim-
dc.date.accessioned2017-04-19T10:10:05Z-
dc.date.available2017-04-19T10:10:05Z-
dc.date.issued2015-
dc.identifier.issn0951-4198-
dc.identifier.uri10.1002/rcm.7157ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12781-
dc.description.abstractRationale In our previous analysis of the matrix-assisted laser desorption/ionization (MALDI) spectra of peptides, we treated their depth profiles in solid samples as homogeneous. Here, we wanted to determine if the reproducible MALDI spectra and linear calibration curves reported previously would be obtained even when the depth profiles were inhomogeneous. Methods We derived a formula relating shot-number-dependent ion abundance data in temperature-controlled MALDI with the analyte depth profile in a solid sample. We prepared samples containing peptides, amino acids, and serotonin in α-cyano-4-hydroxycinnamic acid matrix by vacuum-drying and micro-spotting methods, recorded their MALDI spectra, and analyzed them with the aforementioned formula. Results For the samples prepared by vacuum-drying, the analyte depth profiles were inhomogeneous and maximized at the sample surface. Although the MALDI spectra changed as the shot continued, their sum over the entire set of spectra acquired from a spot was reproducible. Similarly, a high-quality calibration curve could be obtained with the spectral data summed over the entire set. Depth profiles were homogeneous for samples prepared by micro-spotting. Conclusions A method has been developed to obtain a reproducible MALDI spectrum and a linear calibration curve for an analyte with an inhomogeneous depth profile in a solid sample.-
dc.publisherWiley-
dc.titleAcquisition of the depth profiles and reproducible mass spectra in matrix-assisted laser desorption/ionization of inhomogeneous samples-
dc.title.alternativeAcquisition of the depth profiles and reproducible mass spectra in matrix-assisted laser desorption/ionization of inhomogeneous samples-
dc.typeArticle-
dc.citation.titleRapid Communications in Mass Spectrometry-
dc.citation.number8-
dc.citation.endPage752-
dc.citation.startPage745-
dc.citation.volume29-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName안성희-
dc.contributor.alternativeName박경만-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName이성훈-
dc.contributor.alternativeName김명수-
dc.identifier.bibliographicCitationRapid Communications in Mass Spectrometry, vol. 29, no. 8, pp. 745-752-
dc.identifier.doi10.1002/rcm.7157-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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