Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA

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Title
Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA
Author(s)
Yong Beom Shin; Narea Jo; Ki Joong Lee
Bibliographic Citation
Proceedings of SPIE, vol. 9537, pp. 95371F-95371F
Publication Year
2015
Abstract
We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-Antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50-L human serum with limit of detection (LOD) of 0.7 zeptomole (10-21 mole).
Keyword
Biosensorcancer markerdiagnosisELISAimmunosensingLocalized surface plasmon resonancemetal nanodot arraynanoimprintself-controlled
ISSN
0277-786X
Publisher
International Society for Optical Engineering
DOI
http://dx.doi.org/10.1117/12.2183063
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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