Setdb1 is implicated in the methylation of pericentric satellite DNA

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Title
Setdb1 is implicated in the methylation of pericentric satellite DNA
Author(s)
Sunhwa Cho; Jung Sun Park; Y S Jeong; Yong-Kook Kang
Bibliographic Citation
Stem Cell Epigenetics, vol. 2, pp. e938-e938
Publication Year
2015
Abstract
Setdb1/Eset, a histone H3-lysine 9 (H3K9)-specific methyltransferase, interacts with various transcription factors to modify chromatin at target genomic regions. In this study, we provide evidence supporting that Setdb1 also regulates genomic DNA methylation. Whereas 5-methylcytosine (5-mC) signal majorly localizes at pericentric heterochromatin regions in wild type cells, knocking down Setdb1 resulted in diffuse localization of 5-mC signal. Setdb1 knockdown also reduced the expression and subcellular localization of the DNA methyltransferases Dnmt1 and Dnmt3a. Bisulfite mutagenesis studies revealed that Setdb1 knockdown reduced the methylation of satellite DNA sequences (heterochromatin) but not of intracistronic A particle (IAP) sequences (euchromatin). Our results suggest that Setdb1 directly or indirectly regulates the expression of DNA methyltransferases and thus affects DNA methylation and the structural organization of heterochromatin.
Keyword
Setdb1DNA methylationDnmt1Dnmt3Asatellite sequencesIAP
ISSN
2378-3095
Publisher
Smart Science and Technology
DOI
http://dx.doi.org/10.14800/SCE.938
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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