Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers

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dc.contributor.authorEun-Yee Jie-
dc.contributor.authorYoung Bae Ryu-
dc.contributor.authorSeung A Choi-
dc.contributor.authorMyung-Suk Ahn-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorSung Ran Min-
dc.contributor.authorSuk Weon Kim-
dc.date.accessioned2017-04-19T10:12:59Z-
dc.date.available2017-04-19T10:12:59Z-
dc.date.issued2015-
dc.identifier.issn18635466-
dc.identifier.uri10.1007/s11816-015-0369-0ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12881-
dc.description.abstractThe conditions for efficient tuber production from suspension cultures of Pinellia ternata cells which is one of medicinal herbs were established and succinic acid in tubers propagated in vitro was determined. Leaf explants formed white nodular structures and off-white calluses at a frequency of 90.6 % when cultured on Murashige and Skoog medium supplemented with 0.54 μM α-naphthaleneacetic acid (NAA); however, this frequency declined substantially with increasing NAA concentration, up to 16.2 μM, at which the frequency reached zero percent. In combination treatments with 4.44 μM 6-benzyladenine (BA) and NAA, however, the frequency of white nodular structure and off-white callus formation from leaf explants did not decrease, even at 16.2 μM NAA. Suspension cultures of P. ternata cells were established from leaf-derived off-white calluses in MS liquid medium containing 5.4 μM NAA and 4.44 μM BA. Upon plating onto MS basal medium, over 90 % of cell aggregates gave rise to microtubers and developed into plantlets. Regenerated plantlets were transplanted in potting soil and grown to maturity in a growth chamber, with a survival rate of >90 %. The highest succinic acid content in suspension culture-derived microtubers was 45 g/kg of extract. Compared with wild P. ternata medicinal tubers, the succinic acid content was very similar. The in vitro P. ternata microtuber proliferation system established in this study is thus an efficient alternative for the mass production of medicinal tubers.-
dc.publisherSpringer-
dc.titleMass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers-
dc.title.alternativeMass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers-
dc.typeArticle-
dc.citation.titlePlant Biotechnology Reports-
dc.citation.number5-
dc.citation.endPage338-
dc.citation.startPage331-
dc.citation.volume9-
dc.contributor.affiliatedAuthorEun-Yee Jie-
dc.contributor.affiliatedAuthorYoung Bae Ryu-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName지은이-
dc.contributor.alternativeName류영배-
dc.contributor.alternativeName최승아-
dc.contributor.alternativeName안명숙-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName김석원-
dc.identifier.bibliographicCitationPlant Biotechnology Reports, vol. 9, no. 5, pp. 331-338-
dc.identifier.doi10.1007/s11816-015-0369-0-
dc.subject.keywordMicrotuber production-
dc.subject.keywordPinellia ternata-
dc.subject.keywordPlant regeneration-
dc.subject.keywordSuccinic acid-
dc.subject.localMicrotuber production-
dc.subject.localPinellia ternata-
dc.subject.localPlant regeneration-
dc.subject.localSuccinic acid-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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