Cohnella collisoli sp. nov., isolated from lava forest soil

Abstract

A novel bacterial strain, NKM-5T, was isolated from soil of a lava forest in Nokkome Oreum, Jeju, Republic of Korea. Cells of strain NKM-5T were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- and catalase-positive. Strain NKM-5T contained anteiso-C15:0 and iso-C16:0 as the major fatty acids; menaquinone-7 (MK-7) as the predominant isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, an unidentified phospholipid and three unidentified aminophospholipids as the polar lipids; and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 48.3mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain NKM-5T was most closely related to Cohnella lupini RLAHU4BT (96.9% sequence similarity) and fell into a clade in the genus Cohnella. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain NKM-5T represents a novel species of the genus Cohnella, for which the nameCohnella collisoli sp. nov. is proposed. The type strain is NKM-5T (=KCTC 33634T=CECT 8805T).