Pseudoxanthomonas sangjuensis sp. nov., isolated from greenhouse soil

Abstract

One bacterial strain, designated 5GH38-5T, which was characterized as aerobic, Gram-staining-negative, non-flagellated rods, was isolated from a soil sample from a greenhouse in Sangju region, Republic of Korea. It grew at temperatures of 15-45°C, pH5.0-9.0 and NaCl concentrations (w/v) of 0-3.0%. 16S rRNA gene sequence analysis showed the strain was closely related to Pseudoxanthomonas kaohsiungensis J36T (97.3%), Pseudoxanthomonas suwonensis 4M1T (96.8%), Pseudoxanthomonas daejeonensis TR6-08T (96.7%) andPseudoxanthomonas kalamensis JA40T (96.7%). Its major fatty acids were iso-C16:0, anteiso-C15:0 and iso-C15:0. The predominant ubiquinone was Q-8. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 71.1mol%. The DNA-DNA hybridization value between strain 5GH38-5T and P. kaohsiungensis J36T was less than 70%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain 5GH38-5T could be clearly distinguished from closely related members of the genus Pseudoxanthomonas. Therefore, the results of this study indicated the existence of a novel species of the genus Pseudoxanthomonas, for which we propose the name Pseudoxanthomonas sangjuensis sp. nov., with strain 5GH38-5T (=KACC 16961T=DSM 28345T=JCM 19948T) as the type strain.