Development of transgenic strawberry plants expressing monellin, a sweet protein = 감미단백질 모넬린 발현 딸기 형질전환 식물체 개발

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dc.contributor.authorSung Ran Min-
dc.contributor.authorS M Ko-
dc.contributor.authorJae Il Lyu-
dc.contributor.authorJi Hyun Park-
dc.contributor.authorSo Young Yi-
dc.contributor.authorI H Lee-
dc.contributor.authorH S Kim-
dc.contributor.authorT I Kim-
dc.contributor.authorP S Choi-
dc.contributor.authorWon Joong Jeong-
dc.contributor.authorSuk Weon Kim-
dc.contributor.authorJ Kim-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T10:13:35Z-
dc.date.available2017-04-19T10:13:35Z-
dc.date.issued2015-
dc.identifier.issn12292818-
dc.identifier.uri10.5010/JPB.2015.42.3.180ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12934-
dc.description.abstractLeaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.-
dc.publisherSouth Korea-
dc.titleDevelopment of transgenic strawberry plants expressing monellin, a sweet protein = 감미단백질 모넬린 발현 딸기 형질전환 식물체 개발-
dc.title.alternativeDevelopment of transgenic strawberry plants expressing monellin, a sweet protein-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number3-
dc.citation.endPage185-
dc.citation.startPage180-
dc.citation.volume42-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorWon Joong Jeong-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName고석민-
dc.contributor.alternativeName유재일-
dc.contributor.alternativeName박지현-
dc.contributor.alternativeName이소영-
dc.contributor.alternativeName이인하-
dc.contributor.alternativeName김현숙-
dc.contributor.alternativeName김태일-
dc.contributor.alternativeName최필선-
dc.contributor.alternativeName정원중-
dc.contributor.alternativeName김석원-
dc.contributor.alternativeName김종현-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 42, no. 3, pp. 180-185-
dc.identifier.doi10.5010/JPB.2015.42.3.180-
dc.subject.keywordAgrobacterium-mediated transformation-
dc.subject.keywordMonellin-
dc.subject.keywordStrawberry-
dc.subject.keywordSweet protein-
dc.subject.localAgrobacterium-mediated transformation-
dc.subject.localMonellin-
dc.subject.localStrawberry-
dc.subject.localSweet protein-
dc.description.journalClassN-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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