Jatrophihabitans fulvus sp. nov., an actinobacterium isolated from grass soil

Abstract

A Gram-stain-positive, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB158T, was isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the novel isolate in the class Actinobacteria, and most closely related to Jatrophihabitans endophyticus S9-650T and Jatrophihabitans soli KIS75-12T with 98.1 and 97.0% 16S rRNA gene sequence similarity, respectively. Cells of strain PB158T formed yellow colonies on R2A agar, contained MK-9(H4) as the predominant menaquinone, meso-diaminopimelic acid as the diagnostic diamino acid, and included iso-C16: 0, C18: 1ω9c, and C17: 1ω8c as the major fatty acids (> 5%). The acyl type was found to be N-glycolylated. The G+C content of genomic DNA of strain PB158T was 72.4 mol%. In DNA-DNA hybridizations, the DNA-DNA relatedness value observed between strain PB158T and the type strain of J. endophyticus was 21.8% indicating that the two strains do not belong to the same species. Thus, the combined genotypic and phenotypic data supported the conclusion that strain PB158T represents a novel species of the genus Jatrophihabitans, for which the name Jatrophihabitans fulvus sp. nov. is proposed. The type strain is PB158T (=KCTC 33605T=JCM 30448T).