A single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3′-UTR of amplifiable dhfr

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dc.contributor.authorShin Young Kang-
dc.contributor.authorYeon-Gu Kim-
dc.contributor.authorHong-Weon Lee-
dc.contributor.authorEun Gyo Lee-
dc.date.accessioned2017-04-19T10:13:57Z-
dc.date.available2017-04-19T10:13:57Z-
dc.date.issued2015-
dc.identifier.issn01757598-
dc.identifier.uri10.1007/s00253-015-6856-yko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12957-
dc.description.abstractGene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorporating short hairpin RNA (shRNA) that targets the 3′-untranslated region (UTR) of endogenous dhfr. Thus, shRNAs were designed to target the 3′-UTR of endogenous dhfr, and shRNA-2 efficiently down-regulated dhfr expression in CHO-K1 cells. A single gene copy of shRNA-2 also decreased the translational level of DHFR by 80 % in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop EPO-producing cells in the Flp-In system. The specific EPO productivity (qEPO) was enhanced by stepwise increments of MTX concentration, and differences in the amplification rate were observed in Flp-In CHO cells that expressed shRNA-2. In addition, the qEPO increased by more than 2.5-fold in the presence of 500 nM MTX. The mRNA expression level and gene copy numbers of dhfr were correlated with increased productivity in the cells, which is influenced by inhibition of endogenous dhfr. This study reveals that an expression vector including shRNA that targets the 3′-UTR of endogenous dhfr can enhance the transgene amplification rate and productivity by generating DHFR-deficient cells. This approach may be applied for amplifying the foreign gene in wild-type cell lines as a versatile single-plasmid vector.-
dc.publisherSpringer-
dc.titleA single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3′-UTR of amplifiable dhfr-
dc.title.alternativeA single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3′-UTR of amplifiable dhfr-
dc.typeArticle-
dc.citation.titleApplied Microbiology and Biotechnology-
dc.citation.number23-
dc.citation.endPage10126-
dc.citation.startPage10117-
dc.citation.volume99-
dc.contributor.affiliatedAuthorYeon-Gu Kim-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.alternativeName강신영-
dc.contributor.alternativeName김연구-
dc.contributor.alternativeName이홍원-
dc.contributor.alternativeName이은교-
dc.identifier.bibliographicCitationApplied Microbiology and Biotechnology, vol. 99, no. 23, pp. 10117-10126-
dc.identifier.doi10.1007/s00253-015-6856-y-
dc.subject.keywordChinese hamster ovary (CHO)-
dc.subject.keywordDHFR-
dc.subject.keywordGene amplification-
dc.subject.keywordShort hairpin RNA (shRNA)-
dc.subject.keywordUntranslated region (UTR)-
dc.subject.localChinese hamster ovary (CHO)-
dc.subject.localDHFR-
dc.subject.localGene amplification-
dc.subject.localShort hairpin RNA (shRNA)-
dc.subject.localUntranslated region (UTR)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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