Simultaneous determination of 3-O-acetyloleanolic acid and oleanolic acid in rat plasma using liquid chromatography coupled to tandem mass spectrometry

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dc.contributor.authorE Kim-
dc.contributor.authorK Noh-
dc.contributor.authorS J Lee-
dc.contributor.authorB Shin-
dc.contributor.authorJoo Tae Hwang-
dc.contributor.authorSeung Woong Lee-
dc.contributor.authorMun Chual Rho-
dc.contributor.authorW Kang-
dc.date.accessioned2017-04-19T10:14:01Z-
dc.date.available2017-04-19T10:14:01Z-
dc.date.issued2016-
dc.identifier.issn07317085-
dc.identifier.uri10.1016/j.jpba.2015.10.030ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12963-
dc.description.abstract3-. O-Acetyloleanolic acid (OAA) is a triterpenoid compound, and exerts an apoptosis in cancer cell lines, an inhibition of both atopic and allergic contact dermatitis in murine model, and a suppression of inflammatory bone loss in mice. OAA can be converted into oleanolic acid (OA) by hydrolysis in vivo, and OA exhibits several pharmacological effects as well. A liquid chromatographic method using tandem mass spectrometry (MS/MS) was developed for the simultaneous determination of OAA and OA in rat plasma. After liquid-liquid extraction with ethylacetate, both substances were chromatographed on a reversed phase column with a mobile phase of 0.1% formic acid aqueous solution and acetonitrile (1:9, v/v). The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor plasma concentrations of both substances over time following an intravenous administration of OAA in rats.-
dc.publisherElsevier-
dc.titleSimultaneous determination of 3-O-acetyloleanolic acid and oleanolic acid in rat plasma using liquid chromatography coupled to tandem mass spectrometry-
dc.title.alternativeSimultaneous determination of 3-O-acetyloleanolic acid and oleanolic acid in rat plasma using liquid chromatography coupled to tandem mass spectrometry-
dc.typeArticle-
dc.citation.titleJournal of Pharmaceutical and Biomedical Analysis-
dc.citation.number0-
dc.citation.endPage100-
dc.citation.startPage96-
dc.citation.volume118-
dc.contributor.affiliatedAuthorSeung Woong Lee-
dc.contributor.affiliatedAuthorMun Chual Rho-
dc.contributor.alternativeName김은영-
dc.contributor.alternativeName노금한-
dc.contributor.alternativeName이상준-
dc.contributor.alternativeName신범수-
dc.contributor.alternativeName황주태-
dc.contributor.alternativeName이승웅-
dc.contributor.alternativeName노문철-
dc.contributor.alternativeName강원구-
dc.identifier.bibliographicCitationJournal of Pharmaceutical and Biomedical Analysis, vol. 118, pp. 96-100-
dc.identifier.doi10.1016/j.jpba.2015.10.030-
dc.subject.keyword3-O-Acetyloleanolic acid-
dc.subject.keywordLC-MS/MS-
dc.subject.keywordOleanolic acid-
dc.subject.keywordRat plasma-
dc.subject.local3-O-Acetyloleanolic acid-
dc.subject.localLC-MS/MS-
dc.subject.localOleanolic acid-
dc.subject.localRat plasma-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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