The fate of extrachromosomal DNAs in the progeny of plastid-transformed tobacco plants = 엽록체 형질전환 담배의 후대에서 extrachromosomal DNA의 운명

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Title
The fate of extrachromosomal DNAs in the progeny of plastid-transformed tobacco plants = 엽록체 형질전환 담배의 후대에서 extrachromosomal DNA의 운명
Author(s)
Sung Ran Min; S H Jung; Jang Ryol Liu; Won Joong Jeong
Bibliographic Citation
Plant Biotechnology Reports, vol. 9, no. 6, pp. 431-442
Publication Year
2015
Abstract
Plastid transformation is conducted by homologous recombination. Plastid transformation using a vector-containing promoter and/or terminator sequences homologous to the plastid DNA for transgene expression often results in the generation of unintended extrachromosomal DNA molecules derived from the initial plastid DNA molecule via intramolecular recombination. All the extrachromosomal DNA molecules, including those lacking the ori sequence, found in the T0 plastid-transformed tobacco plants were still detected in the T3 plants. These results suggest that the extrachromosomal DNA molecules are newly generated from the initially transformed plastid DNA that is transmitted to the progeny during each generation. In addition, inward, outward, and overlap extension PCR of the extrachromosomal DNA molecules confirmed that these molecules were circular.
Keyword
Extrachromosomal DNAHomologous recombinationPlastid transformationTobacco
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-015-0380-5
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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