Donghicola tyrosinivorans sp. nov., a tyrosine-degrading bacterium isolated from seawater = 해수에서 분리한 tyrosine 분해 신종 세균 Donghicola tyrosinivorans

Abstract

A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1T, was isolated from seawater off Jeju Island, Republic of Korea and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. This strain catalase-positive, oxidase-negative and grew optimally at 30 ºC, in the presence of 1-2% (w/v) NaCl and at pH 7.0-7.5. It did not synthesize bacteriochlorophyll a. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1T clustered with the type strain Donghicola eburneus KCTC 12735T (97.0% 16S rRNA gene sequence similarity). DNA-DNA hybridization value between strain 13-93-B1T and strain D. eburneus KCTC 12735T was 33.1±1.4% (35.2±2.8% in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (C18:1ω7c/C18:1ω6c, 76.9%). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1T was 60.4 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1T is considered to represent a novel species of the genus Donghicola, for which the name Donghicola tyrosinivorans sp. nov. is proposed. The type strain is 13-93-B1T (=DSM 100212T=KCTC 42571T).