Bacterial RNAs activate innate immunity in Arabidopsis

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Bacterial RNAs activate innate immunity in Arabidopsis
Boyoung Lee; Yong-Soon Park; Soohyun Lee; Geun Cheol Song; Choong-Min Ryu
Bibliographic Citation
New Phytologist, vol. 209, no. 2, pp. 785-797
Publication Year
The common molecular patterns of microbes play a critical role in the regulation of plant innate immunity. However, little is known about the role of nucleic acids in this process in plants. We pre-infiltrated Arabidopsis leaves with total RNAs from Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) and subsequently inoculated these plants with the same bacterial cells. Total Pto DC3000 RNAs pre-infiltrated into Arabidopsis leaves elicited plant immune responses against Pto DC3000. However, sheared RNAs and RNase A application failed to induce immunity, suggesting that intact bacterial RNAs function in plant innate immunity. This notion was supported by the positive regulation of superoxide anion levels, callose deposition, two mitogen-activated protein kinases and defense-related genes observed in bacterial RNA-pre-treated leaves. Intriguingly, the Pto DC3000 population was not compromised in known pattern recognition receptor mutants for chitin, flagellin and elongation factor-Tu (EF-Tu). Plant defense-related mutant analyses further revealed that bacterial RNA-elicited innate immunity was normally required for salicylic and jasmonic acid signaling. Notably, among total RNAs, the abundant bacterial RNA species 16S and 23S ribosomal RNAs were the major determinants of this response. Our findings provide evidence that bacterial RNA serves as a microbe-associated molecular pattern in plants.
Pseudomonas syringaeBacterial RNAsJasmonic acid (JA)Microbe-associated molecular pattern (MAMP)Plant innate immunitySalicylic acid (SA)
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Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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