Protective effects of new blackberry cultivar MNU-32 extracts against H2O2-induced oxidative stress in HepG2 cells

Abstract

Protective effects of extracts of the new cultivar MNU-32 developed from blackberry (Rubus fruticosus) cultivar V-9 against H2O2-induced oxidative stress in HepG2 cells were investigated. DPPH radical scavenging activities of MNU-32 and V-9 extracts were 570.00 μg/mL (IC50) and 644.55 μg/mL (IC50), respectively. Inhibition effects of MNU-32 extracts against intracellular ROS generation, cell cytotoxicity, and lactate dehydrogenase release were higher than for the V-9 extract in H2O2-treated HepG2 cells. Compared with the V-9 extract, MNU-32 extract treatments produced higher levels of lipid peroxidation attenuation and greater protection against DNA damage, restored the activity of superoxide dismutase in H2O2-treated HepG2 cells, and increased protein expression levels of superoxide dismutase in HepG2 cells in a dose and time-dependent manner. MNU-32 blackberry extracts exerted protective effects against H2O2-induced cell damage due to higher anthocyanin contents than the V-9 blackberry extract.