Establishment and maintenance of an axenic culture of Ettlia sp. using a species-specific approach

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dc.contributor.authorHyung Gwan Lee-
dc.contributor.authorSang Yoon Shin-
dc.contributor.authorL Jin-
dc.contributor.authorChan Yoo-
dc.contributor.authorA Srivastava-
dc.contributor.authorHyun Joon La-
dc.contributor.authorChi-Yong Ahn-
dc.contributor.authorHee-Sik Kim-
dc.contributor.authorHee-Mock Oh-
dc.date.accessioned2017-04-19T10:16:17Z-
dc.date.available2017-04-19T10:16:17Z-
dc.date.issued2015-
dc.identifier.issn12268372-
dc.identifier.uri10.1007/s12257-015-0289-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13066-
dc.description.abstractThe establishment of an axenic culture of microalgae is essential step in understanding its physiology, genetics, and ecology. However, culturing of microalgae is usually accompanied by complex and variable associated prokaryotic and eukaryotic microorganisms. Conventional approaches used for obtaining axenic cultures of microalgae are time-consuming and often involve difficulties in maintaining and preserving axenicity. In this study, we developed a procedure for establishing an axenic culture of Ettlia sp. YC001 and demonstrate that we maintained the axenic culture through subculture in the long term. Three sequential treatments, an antibiotic cocktail, serial dilution, and plate spreading, were applied to strain YC001 and we confirmed axenicity using molecular and physiological methods. The bacterial community associated with strain YC001 was investigated to select antibiotics for their specific elimination. The xenic culture (1 × 106 cells/mL) was treated with the antibiotic cocktail-5 (AC-5), carbendazim, chloramphenicol, imipenem, rifampicin, and tetracycline for 3 days, followed by serial dilution up to 1 × 102 cells and spreading on agar plates. The pure colonies were analyzed using denaturing gradient gel electrophoresis (DGGE), fluorescence-activated cell sorting (FACS), and scanning electron microscopy (SEM). The procedure we developed can be applied to other strains of microalgae for the establishment of axenic cultures.-
dc.publisherSouth Korea-
dc.titleEstablishment and maintenance of an axenic culture of Ettlia sp. using a species-specific approach-
dc.title.alternativeEstablishment and maintenance of an axenic culture of Ettlia sp. using a species-specific approach-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number6-
dc.citation.endPage1063-
dc.citation.startPage1056-
dc.citation.volume20-
dc.contributor.affiliatedAuthorHyung Gwan Lee-
dc.contributor.affiliatedAuthorChi-Yong Ahn-
dc.contributor.affiliatedAuthorHee-Sik Kim-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.alternativeName이형관-
dc.contributor.alternativeName신상윤-
dc.contributor.alternativeNameJin-
dc.contributor.alternativeName유찬-
dc.contributor.alternativeName안키타-
dc.contributor.alternativeName나현준-
dc.contributor.alternativeName안치용-
dc.contributor.alternativeName김희식-
dc.contributor.alternativeName오희목-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 20, no. 6, pp. 1056-1063-
dc.identifier.doi10.1007/s12257-015-0289-4-
dc.subject.keywordantibiotic cocktail-
dc.subject.keywordaxenic culture-
dc.subject.keywordEttlia sp. YC001-
dc.subject.keywordplate spreading-
dc.subject.keywordserial dilution-
dc.subject.localantibiotic cocktail-
dc.subject.localaxenic culture-
dc.subject.localAxenic culture-
dc.subject.localEttlia sp. YC001-
dc.subject.localplate spreading-
dc.subject.localserial dilution-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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