Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural

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Title
Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural
Author(s)
H M Seo; J M Jeon; J H Lee; H S Song; H B Joo; S H Park; K Y Choi; Yong Hyun Kim; K Park; Jungoh AhnHong-Weon Lee; Y H Yang
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 43, no. 1, pp. 37-44
Publication Year
2016
Abstract
Furfural is a toxic by-product formulated from pretreatment processes of lignocellulosic biomass. In order to utilize the lignocellulosic biomass on isobutanol production, inhibitory effect of the furfural on isobutanol production was investigated and combinatorial application of two oxidoreductases, FucO and YqhD, was suggested as an alternative strategy. Furfural decreased cell growth and isobutanol production when only YqhD or FucO was employed as an isobutyraldehyde oxidoreductase. However, combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol production by 110 % compared with overexpression of YqhD. The combinatorial oxidoreductases increased furfural detoxification rate 2.1-fold and also accelerated glucose consumption 1.4-fold. When it compares to another known system increasing furfural tolerance, membrane-bound transhydrogenase (pntAB), the combinatorial aldehyde oxidoreductases were better on cell growth and production. Thus, to control oxidoreductases is important to produce isobutanol using furfural-containing biomass and the combinatorial overexpression of FucO and YqhD can be an alternative strategy.
Keyword
Aldehyde oxidoreductaseEscherichia coliFurfuralIsobutanol
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-015-1718-2
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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