Cited 18 time in
- Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural
- H M Seo; J M Jeon; J H Lee; H S Song; H B Joo; S H Park; K Y Choi; Yong Hyun Kim; K Park; Jungoh Ahn; Hong-Weon Lee; Y H Yang
- Bibliographic Citation
- Journal of Industrial Microbiology & Biotechnology, vol. 43, no. 1, pp. 37-44
- Publication Year
- Furfural is a toxic by-product formulated from pretreatment processes of lignocellulosic biomass. In order to utilize the lignocellulosic biomass on isobutanol production, inhibitory effect of the furfural on isobutanol production was investigated and combinatorial application of two oxidoreductases, FucO and YqhD, was suggested as an alternative strategy. Furfural decreased cell growth and isobutanol production when only YqhD or FucO was employed as an isobutyraldehyde oxidoreductase. However, combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol production by 110 % compared with overexpression of YqhD. The combinatorial oxidoreductases increased furfural detoxification rate 2.1-fold and also accelerated glucose consumption 1.4-fold. When it compares to another known system increasing furfural tolerance, membrane-bound transhydrogenase (pntAB), the combinatorial aldehyde oxidoreductases were better on cell growth and production. Thus, to control oxidoreductases is important to produce isobutanol using furfural-containing biomass and the combinatorial overexpression of FucO and YqhD can be an alternative strategy.
- Aldehyde oxidoreductase; Escherichia coli; Furfural; Isobutanol
- Appears in Collections:
- Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.