Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes: greater effect on metabolites in PBMC than plasma

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Title
Replacing carbohydrate with protein and fat in prediabetes or type-2 diabetes: greater effect on metabolites in PBMC than plasma
Author(s)
M Kim; G Song; M Kang; H J Yoo; Tae Sook Jeong; S H Lee; J H Lee
Bibliographic Citation
Nutrition & Metabolism, vol. 13, pp. 3-3
Publication Year
2016
Abstract
Background: Active metabolism of peripheral blood mononuclear cells (PBMC) could suggest their suitability for metabolomics studies. This study examined whether reductions in PBMCs and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activities induced by dietary intervention affected the overall metabolic profiles of PBMC and plasma. Methods: Eighty nonobese subjects aged 40-70 years (18.5 & BMI < 30 kg/mbsupesup) with prediabetes or newly-diagnosed type-2 diabetes were assigned to consume either the usual refined-rice diet (control group, n = 40) or to replace refined rice with whole grains and legumes as carbohydrates (whole-grain group, n = 40) for three meals per day during the 12-week intervention. Fasting PBMC and plasma metabolomes were profiled using UPLC-LTQ-Orbitrap mass spectrometry. Results: After 12 weeks, changes in fasting glucose, HbAbsubesub, HOMA-IR, MDA, ox-LDL, LDL particle size, plasma Lp-PLA bsubesub activity, and PBMC enzyme activity in the whole-grain group were significantly different from those in the control group before and after adjusting for baseline levels. The PBMC levels of L-leucine, oleamide, lysoPC (16:0), and lysoPC (18:0) in the whole-grain group showed greater reductions compared with those of the control group. Changes in plasma metabolites were not significantly different between the two groups. Changes in PBMC Lp-PLAbsubesub activity positively correlated with changes in L-leucine, oleamide, lysoPC (16:0), lysoPC (18:0), glucose, and ox-LDL, and negatively correlated with changes in LDL particle size. Conclusions: This study showed that dietary intervention in prediabetic or type-2 diabetic patients had a greater effect on PBMC Lp-PLAbsubesub activity and metabolites compared with those of plasma metabolites.
Keyword
MetabolitesPeripheral blood mononuclear cellsPrediabetesWhole-grains and legumes
ISSN
1743-7075
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s12986-016-0063-4
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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