Development of an efficient In vitro screening method for selection of resistan lily cutivars against Fusarium oxysporum f. sp. lilii = 백합 구근부패병 저항성 검정을 위한 기내 간편 검정법 개발

Cited 2 time in scopus
Metadata Downloads
Title
Development of an efficient In vitro screening method for selection of resistan lily cutivars against Fusarium oxysporum f. sp. lilii = 백합 구근부패병 저항성 검정을 위한 기내 간편 검정법 개발
Author(s)
Ji Young Jang; Ki Beom Moon; Jang Ho Ha; Ji Sun Park; Mi-Jin Kim; Jae Heung Jeon; G J Lee; Hyun Soon Kim
Bibliographic Citation
Horticultural Science & Technology, vol. 33, no. 6, pp. 883-890
Publication Year
2015
Abstract
The soilborne fungus Fusarium oxysporum f. sp. lilii (Fol) is a serious threat to all lily cultivars, especially infecting bulbs and flowers. It has become increasingly important to develop varieties resistant against the bulb rot disease. Genetic diversity of cultivars and reliable screening methods are required for this purpose. Here, an efficient in vitro screening system for evaluating resistance to Fol in 38 in vitro-grown lily plants was investigated. Various factors including culture conditions of Fol, inoculum density, appropriate plant materials, inoculation method and duration, and incubation period of plant materials after inoculation were combined to optimize the screening method. As a result, we suggest optimal conditions for an in vitro screening system for the selection of Fol-resistant lily cultivars as follows. Fol was grown on potato dextrose agar (PDA) medium for 6 days at 25°C in darkness and used as working inoculation. Spore suspensions were prepared (inoculum density: 1.0 × 104 spores?mL-1), and then leaf segments 1.5 × 2.0 cm2 were inoculated by dipping for 22 hours at 25°C in dark. Later, leaves were cultured on 0.6% agar plates at 25°C and 50% humidity with a photoperiod of 16 hours light/8 hours dark (fluence rate of 40 μmol?m-2 ?s-1) to examine the progress of bulb rot. After 7 days, disease levels were classified into indices 1 (no symptom) to 6 (serious bulb rot). Soil inoculation of Fol carried out with resistant or susceptible lily cultivars that had been selected through in vitro screening confirmed the reproducibility of results. Therefore, the in vitro screening method established in this study is efficient and reliable for selection of lily cultivars resistant against bulb rot disease.
Keyword
bulb rotdisease indexdisease resistanceLillium spp.
ISSN
1226-8763
Publisher
South Korea
DOI
http://dx.doi.org/10.7235/hort.2015.15085
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.