Identification of haplotypes at the Rsv4 genomic region in soybean associated with durable resistance to soybean mosaic virus = 콩 모자? 바이러스의 내구 저항성과 연관된 콩 Rsv4 유전체 부위 일배체의 동정

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Title
Identification of haplotypes at the Rsv4 genomic region in soybean associated with durable resistance to soybean mosaic virus = 콩 모자? 바이러스의 내구 저항성과 연관된 콩 Rsv4 유전체 부위 일배체의 동정
Author(s)
D C Ilut; A E Lipka; Namhee Jeong; Dong Nyuk Bae; Dong Hyun Kim; Ji Hong Kim; N Redekar; Kiwoong Yang; Won Park; S T Kang; Namshin Kim; J K Moon; M A S Maroof; M A Gore; Soon Chun Jeong
Bibliographic Citation
Theoretical and Applied Genetics, vol. 129, no. 3, pp. 453-468
Publication Year
2016
Abstract
The Soybean mosaic virus (SMV) resistance locus Rsv4 is of interest because it provides a durable type of resistance in soybean [Glycine max (L.) Merr.]. To better understand its molecular basis, we used a population of 309 BC3F2 individuals to fine-map Rsv4 to a ~120 kb interval and leveraged this genetic information in a second study to identify accessions ‘Haman’ and ‘Ilpumgeomjeong’ as new sources of Rsv4. These two accessions along with three other Rsv4 and 14 rsv4 accessions were used to examine the patterns of nucleotide diversity at the Rsv4 region based on high-depth resequencing data. Through a targeted association analysis of these 19 accessions within the ~120 kb interval, a cluster of four intergenic single-nucleotide polymorphisms (SNPs) was found to perfectly associate with SMV resistance. Interestingly, this ~120 kb interval did not contain any genes similar to previously characterized dominant disease resistance genes. Therefore, a haplotype analysis was used to further resolve the association signal to a ~94 kb region, which also resulted in the identification of at least two Rsv4 haplotypes. A haplotype phylogenetic analysis of this region suggests that the Rsv4 locus in G. max is recently introgressed from G. soja. This integrated study provides a strong foundation for efforts focused on the cloning of this durable virus resistance gene and marker-assisted selection of Rsv4-mediated SMV resistance in soybean breeding programs
ISSN
0040-5752
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00122-015-2640-8
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
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