Highly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L. = Helianthus tuberosus의 고효율 재분화 및 아그로박테리움-매개 형질전환

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dc.contributor.authorMee Jin Kim-
dc.contributor.authorDong Ju An-
dc.contributor.authorKi Beom Moon-
dc.contributor.authorHye Sun Cho-
dc.contributor.authorSung Ran Min-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorHyun Soon Kim-
dc.date.accessioned2017-04-19T10:19:35Z-
dc.date.available2017-04-19T10:19:35Z-
dc.date.issued2016-
dc.identifier.issn09266690-
dc.identifier.uri10.1016/j.indcrop.2015.12.054ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13207-
dc.description.abstractIn our current study, to develop an efficient regeneration and transformation system of Helianthus tuberosus, we verified effects of plant growth hormones, growth conditions, explant type, and transformation conditions. Leaf segments from regenerated shoots showed higher regeneration efficiency on MS basal medium containing 1.0mgL-1 zeatin under darkness than those from maintained in vitro plant. To carry out transformation, various parameters including plant materials, Agrobacterium cell density, immersion time, and Agrobacterium strains (leaf segment, OD600-0.6, 60min, and Agrobacterium tumefaciens LBA4404 harboring binary vector pCAMBIA1301) have been determined. The putatively transformed H. tuberosus were screened by survival rate and beta-glucuronidase (GUS) histochemical assay following two cycles of 3.0mgL-1 hygromycin selection at callus stage. The presence of the selectable marker gene hygromycin phosphotransferase and the GUS reporter gene with intron was then confirmed by genomic PCR, Southern blot, reverse transcriptase PCR (RT-PCR) and GUS histochemical assay. The method presented here could be helpful in genetic improvement of H. tuberosus through efficient shoot regeneration and stable Agrobacterium-mediated transformation.-
dc.publisherElsevier-
dc.titleHighly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L. = Helianthus tuberosus의 고효율 재분화 및 아그로박테리움-매개 형질전환-
dc.title.alternativeHighly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L.-
dc.typeArticle-
dc.citation.titleIndustrial Crops and Products-
dc.citation.number0-
dc.citation.endPage679-
dc.citation.startPage670-
dc.citation.volume83-
dc.contributor.affiliatedAuthorHye Sun Cho-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorHyun Soon Kim-
dc.contributor.alternativeName김미진-
dc.contributor.alternativeName안동주-
dc.contributor.alternativeName문기범-
dc.contributor.alternativeName조혜선-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName김현순-
dc.identifier.bibliographicCitationIndustrial Crops and Products, vol. 83, pp. 670-679-
dc.identifier.doi10.1016/j.indcrop.2015.12.054-
dc.subject.keywordJerusalem artichoke-
dc.subject.keywordRegenerated plants-
dc.subject.keywordShoot regeneration-
dc.subject.keywordStable transformation-
dc.subject.localJerusalem artichoke-
dc.subject.localRegenerated plants-
dc.subject.localShoot regeneration-
dc.subject.localStable transformation-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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