Paenibacillus cathormii sp. nov., isolated from tree bark

Abstract

A Gram-stain-positive, facultatively anaerobic, rod-shaped and endospore-forming bacterium, strain BK114-2T isolated from tree bark in Thailand was characterized taxonomically using a polyphasic approach. Analysis based on comparison of 16S rRNA gene sequences indicated that strain BK114-2T was affiliated to the genus Paenibacillus, and was closely related to Paenibacillus timonensis 2301032T (96.7% 16S rRNA gene sequence similarity), Paenibacillus phoenicis 3PO2SAT (96.6%) and Paenibacillus barengoltzii SAFN-016T (96.4%). Strain BK114-2T contained meso-diaminopimelic acid in its cell-wall peptidoglycan. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unknown phospholipids, five unknown aminophospholipids and six unknown lipids. The only menaquinone detected was MK-7 and the dominant cellular fatty acids were C16: 0 (22.9%), anteiso-C15: 0 (22.6%), iso-C16: 0 (19.4%) and anteiso-C17: 0 (14.7%). The DNA G+C content was 52.0 mol%. Based on these results, strain BK114-2T repreesents a novel species of the genus Paenibacillus, for which the name Paenibacillus cathormii sp. nov. is proposed. The type strain is BK114-2T (=KCTC 33251T=TISTR 2282T).