The HIST1 locus escapes reprogramming in cloned bovine embryos

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Title
The HIST1 locus escapes reprogramming in cloned bovine embryos
Author(s)
ByungKuk Min; Sunwha Cho; Jung Sun Park; Kyuheum Jeon; Yong-Kook Kang
Bibliographic Citation
G3-Genes Genomes Genetics, vol. 6, no. 5, pp. 1365-1371
Publication Year
2016
Abstract
Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We observed that histone genes residing in the 1.5 Mb spanning the cow HIST1 cluster were coordinately downregulated in SCNT blastocysts. In contrast, both the nonhistone genes of this cluster, and histone genes elsewhere remained unaffected. This indicated that the downregulation was specific to HIST1 histone genes. We found that, after trichostatin A treatment, HIST1 histone genes were derepressed, and DNA methylation at their promoters was decreased to the level of in vitro fertilization embryos. Therefore, our results indicate that the reduced expression of HIST1 histone genes is a consequence of poor epigenetic reprogramming in SCNT blastocysts.
Keyword
DNA methylationEpigeneticsHDAC inhibitorHIST1 clusterReprogrammingRNA-seqSCNT
ISSN
2160-1836
Publisher
Genetics Society America
DOI
http://dx.doi.org/10.1534/g3.115.026666
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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