Antiviral activity of micafungin against enterovirus 71 = 미카펀진의 장바이러스 억제효능

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Title
Antiviral activity of micafungin against enterovirus 71 = 미카펀진의 장바이러스 억제효능
Author(s)
C Kim; Hyunju Kang; Dong-eun Kim; J H Song; Miri Choi; Mingu Kang; K Lee; H S Kim; J S Shin; Hyejeong Jeong; Sunhee Jung; S B Han; J H Kim; H J Ko; C K Lee; M Kim; Sungchan Cho
Bibliographic Citation
Virology Journal, vol. 13, pp. 99-99
Publication Year
2016
Abstract
Background: Enterovirus 71 (EV71) is a major causative agent of hand-foot-mouth disease (HFMD) and also causes severe neurological complications, leading to fatality in young children. However, no effective therapy is currently available for the treatment of this infection. Methods: We identified small-molecule inhibitors of EV71 from a screen of 968 Food and Drug Administration (FDA)-approved drugs, with which clinical application for EV71-associated diseases would be more feasible, using EV71 subgenomic replicon system. Primary hits were extensively evaluated for their antiviral activities in EV71-infected cells. Results: We identified micafungin, an echinocandin antifungal drug, as a novel inhibitor of EV71. Micafungin potently inhibits the proliferation of EV71 as well as the replication of EV71 replicon in cells with a low micromolar IC50 (~5 μM). The strong antiviral effect of micafungin on EV71 replicon and the result from time-of-addition experiment demonstrated a targeting of micafungin on virion-independent intracellular process(es) during EV71 infection. Moreover, an extensive analysis excluded the involvement of 2C and 3A proteins, IRES-dependent translation, and also that of polyprotein processing in the antiviral effect of micafungin. Conclusions: Our research revealed a new indication of micafungin as an effective inhibitor of EV71, which is the first case reporting antiviral activity of micafungin, an antifungal drug.
Keyword
EnterovirusEnterovirus 71 (EV71)MicafunginFDA-approved drugAntiviral drug
ISSN
1743-422X
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s12985-016-0557-8
Type
Article
Appears in Collections:
Ochang Branch Institute > Nucleic Acid Therapeutics Research Center > 1. Journal Articles
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