DC Field | Value | Language |
---|---|---|
dc.contributor.author | Joon Hui Chung | - |
dc.contributor.author | A Bhat | - |
dc.contributor.author | Chang-Jin Kim | - |
dc.contributor.author | D Yong | - |
dc.contributor.author | Choong-Min Ryu | - |
dc.date.accessioned | 2017-04-19T10:23:19Z | - |
dc.date.available | 2017-04-19T10:23:19Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | 10.1038/srep28168 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/13305 | - |
dc.description.abstract | Polymyxins are last-resort antibiotics for treating infections of Gram-negative bacteria. The recent emergence of polymyxin-resistant bacteria, however, urgently demands clinical optimisation of polymyxin use to minimise further evolution of resistance. In this study we developed a novel combination therapy using minimal concentrations of polymyxin B. After large-scale screening of Streptomyces secondary metabolites, we identified a reliable polymixin synergist and confirmed as netropsin using high-pressure liquid chromatography, nuclear magnetic resonance, and mass spectrometry followed by in vitro assays using various Gram-negative pathogenic bacteria. To evaluate the effectiveness of combining polymixin B and netropsin in vivo, we performed survival analysis on greater wax moth Galleria mellonella infected with colistin-resistant clinical Acinetobacter baumannii isolates as well as Escherichia coli, Shigella flexineri, Salmonella typhimuruim, and Pseudomonas aeruginosa. The survival of infected G. mellonella was significantly higher when treated with polymyxin B and netropsin in combination than when treated with polymyxin B or netropsin alone. We propose a netropsin combination therapy that minimises the use of polymyxin B when treating infections with multidrug resistant Gram-negative bacteria. | - |
dc.publisher | Springer-Nature Pub Group | - |
dc.title | Combination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii | - |
dc.title.alternative | Combination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii | - |
dc.type | Article | - |
dc.citation.title | Scientific Reports | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 28168 | - |
dc.citation.startPage | 28168 | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Joon Hui Chung | - |
dc.contributor.affiliatedAuthor | Chang-Jin Kim | - |
dc.contributor.affiliatedAuthor | Choong-Min Ryu | - |
dc.contributor.alternativeName | 정준휘 | - |
dc.contributor.alternativeName | Bhat | - |
dc.contributor.alternativeName | 김창진 | - |
dc.contributor.alternativeName | 용동은 | - |
dc.contributor.alternativeName | 류충민 | - |
dc.identifier.bibliographicCitation | Scientific Reports, vol. 6, pp. 28168-28168 | - |
dc.identifier.doi | 10.1038/srep28168 | - |
dc.description.journalClass | Y | - |
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