Combination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii

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dc.contributor.authorJoon Hui Chung-
dc.contributor.authorA Bhat-
dc.contributor.authorChang-Jin Kim-
dc.contributor.authorD Yong-
dc.contributor.authorChoong-Min Ryu-
dc.date.accessioned2017-04-19T10:23:19Z-
dc.date.available2017-04-19T10:23:19Z-
dc.date.issued2016-
dc.identifier.issn2045-2322-
dc.identifier.uri10.1038/srep28168ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13305-
dc.description.abstractPolymyxins are last-resort antibiotics for treating infections of Gram-negative bacteria. The recent emergence of polymyxin-resistant bacteria, however, urgently demands clinical optimisation of polymyxin use to minimise further evolution of resistance. In this study we developed a novel combination therapy using minimal concentrations of polymyxin B. After large-scale screening of Streptomyces secondary metabolites, we identified a reliable polymixin synergist and confirmed as netropsin using high-pressure liquid chromatography, nuclear magnetic resonance, and mass spectrometry followed by in vitro assays using various Gram-negative pathogenic bacteria. To evaluate the effectiveness of combining polymixin B and netropsin in vivo, we performed survival analysis on greater wax moth Galleria mellonella infected with colistin-resistant clinical Acinetobacter baumannii isolates as well as Escherichia coli, Shigella flexineri, Salmonella typhimuruim, and Pseudomonas aeruginosa. The survival of infected G. mellonella was significantly higher when treated with polymyxin B and netropsin in combination than when treated with polymyxin B or netropsin alone. We propose a netropsin combination therapy that minimises the use of polymyxin B when treating infections with multidrug resistant Gram-negative bacteria.-
dc.publisherSpringer-Nature Pub Group-
dc.titleCombination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii-
dc.title.alternativeCombination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii-
dc.typeArticle-
dc.citation.titleScientific Reports-
dc.citation.number0-
dc.citation.endPage28168-
dc.citation.startPage28168-
dc.citation.volume6-
dc.contributor.affiliatedAuthorJoon Hui Chung-
dc.contributor.affiliatedAuthorChang-Jin Kim-
dc.contributor.affiliatedAuthorChoong-Min Ryu-
dc.contributor.alternativeName정준휘-
dc.contributor.alternativeNameBhat-
dc.contributor.alternativeName김창진-
dc.contributor.alternativeName용동은-
dc.contributor.alternativeName류충민-
dc.identifier.bibliographicCitationScientific Reports, vol. 6, pp. 28168-28168-
dc.identifier.doi10.1038/srep28168-
dc.description.journalClassY-
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Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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