Development and evaluation of species-specific PCR for detection of nine acinetobacter species

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Title
Development and evaluation of species-specific PCR for detection of nine acinetobacter species
Author(s)
X M Li; J A Choi; I S Choi; J K Kook; Young Hyo Chang; G Park; S J Jang; S H Kang; D S Moon
Bibliographic Citation
Annals of Clinical and Laboratory Science, vol. 46, no. 3, pp. 270-278
Publication Year
2016
Abstract
Molecular methods have the potential to improve the speed and accuracy of Acinetobacter species identification in clinical settings. The goal of this study is to develop species-specific PCR assays based on differences in the RNA polymerase beta-subunit gene (rpoB) to detect nine commonly isolated Acinetobacter species including Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter pittii, Acinetobacter nosocomialis, Acinetobacter lwoffii, Acinetobacter ursingii, Acinetobacter bereziniae, Acinetobacter haemolyticus, and Acinetobacter schindleri. The sensitivity and specificity of these nine assays were measured using genomic DNA templates from 55 reference strains and from 474 Acinetobacter clinical isolates. The sensitivity of A. baumannii-specific PCR assay was 98.9%, and the sensitivity of species-specific PCR assays for all other species was 100%. The specificities of A. lwoffii- and A. schindleri-specific PCR were 97.8 and 98.9%, respectively. The specificity of species-specific PCR for all other tested Acinetobacter species was 100%. The lower limit of detection for the nine species-specific PCR assays developed in this study was 20 or 200 pg of genomic DNA from type strains of each species . The Acinetobacter species-specific PCR assay would be useful to determine the correct species among suggested candidate Acinetobacter species when conventional methods including MALDI-TOF MS identify Acinetobacter only to the genus level. The species-specific assay can be used to screen large numbers of clinical and environmental samples obtained for epidemiologic study of Acinetobacter for the presence of target species. ⓒ 2016 by the Association of Clinical Scientists, Inc.
Keyword
AcinetobacterDNA primersLimit of detectionPolymerase chain reactionRpoB geneSensitivity and specificitySpecies
ISSN
0091-7370
Publisher
Assoc Clinical Scientists
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Infrastructure Policy Support Center > 1. Journal Articles
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