Cited 20 time in
- Genome-wide DNA methylation profiles altered by Helicobacter pylori in gastric mucosa and blood leukocyte DNA
- Y Zhang; X R Zhang; Jong Lyul Park; Jong Hwan Kim; L Zhang; J L Ma; W D Liu; D J Deng; W C You; Yong Sung Kim; K F Pan
- Bibliographic Citation
- Oncotarget, vol. 7, no. 24, pp. 37132-37144
- Publication Year
- Purpose: To investigate Helicobacter pylori (H.pylori) associated genomewide
aberrant methylation patterns in gastric mucosa and blood leukocyte DNA, a
population-based study was conducted in Linqu County.
Results: A total of 3000 and 386 CpGs were differentially methylated after
successful H.pylori eradication in gastric mucosa and blood leukocyte DNA respectively,
and 17 were the same alteration trend in the both tissues. The differentially
methylated CpGs were located more frequently in promoters or CpG islands for gastric
mucosa and gene body or open sea for blood leukocyte DNA. In eradicated gastric
mucosa, the hypermethylated CpGs were enriched across inflammatory pathways,
while the hypomethylated CpGs in tube morphogenesis, development and so on. The
final validation found lower SPI1, PRIC285 and S1PR4 methylation levels in H.pylori
positive subjects by case-control comparison, and increased methylation levels in
H.pylori eradicated gastric mucosa by self-comparison. The Cancer Genome Atlas
(TCGA) database analysis suggested that the up-regulation of the three genes by
hypomethylation might be associated with gastric carcinogenesis.
Experimental Design: Infinium HumanMethylation 450K BeadChip was used to
compare methylation profiles prior to and after eradication treatment. The methylation
levels of identified candidate differentially methylated genes before and after H.pylori
eradication were further validated by two stages (Stage I: self-comparison of 16
subjects before and after anti-H.pylori treatment; Stage II: case-control comparison
of 25 H.pylori positive and 25 negative subjects and self-comparison of 50 anti-H.
pylori treated subjects).
Conclusions: Novel H.pylori associated aberrant methylated genes were identified
across the whole genome both in gastric mucosa and blood leukocyte DNA.
- Blood leukocyteGastric mucosaMethylation arrayH. pylori
- Impact Journals
- Appears in Collections:
- Aging Convergence Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.