Characterization of mammalian ADAM2 and its absence from human sperm = 사람의 정자에서 포유류 ADAM2의 특성 분석

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Title
Characterization of mammalian ADAM2 and its absence from human sperm = 사람의 정자에서 포유류 ADAM2의 특성 분석
Author(s)
H Choi; S Jin; J T Kwon; J Kim; J Jeong; J Kim; S Jeon; Z Y Park; Kang Jin Jeong; K Park; C Cho
Bibliographic Citation
PLoS One, vol. 11, no. 6, pp. e0158321-e0158321
Publication Year
2016
Abstract
members of the ADAM (a disintegrin and metalloprotease) family are membraneanchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.
ISSN
1932-6203
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pone.0158321
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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