Ganglioside GD1a promotes oocyte maturation, furthers preimplantation development, and increases blastocyst quality in pigs

Cited 7 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ W Kim-
dc.contributor.authorH J Park-
dc.contributor.authorS K Chae-
dc.contributor.authorJ H Ahn-
dc.contributor.authorG Y Do-
dc.contributor.authorY K Choo-
dc.contributor.authorJ J Park-
dc.contributor.authorB D Jung-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorD B Koo-
dc.date.accessioned2017-04-19T10:24:50Z-
dc.date.available2017-04-19T10:24:50Z-
dc.date.issued2016-
dc.identifier.issnI000-0068-
dc.identifier.uri10.1262/jrd.2015-083ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13334-
dc.description.abstractGangliosides are key lipid molecules required for the regulation of cellular processes such as proliferation, differentiation, and cell signaling, including signaling of epidermal growth factor receptor (EGFR). Epidermal growth factor (EGF) has long been considered a potential regulator of meiotic and cytoplasmic maturation in mammalian oocytes. However, there is no report on the direct effect of ganglioside GD1a in porcine oocyte maturation. In this study, we first investigated a functional link between GD1a and meiotic maturation during in vitro maturation (IVM) of porcine embryos. Moreover, we confirmed the effect of exogenous GD1a treatment on blastocyst development, quality, and fertilization rate in early embryonic development. First, we observed that the protein level of ST3GAL2, a GD1a synthesizing enzyme, significantly increased (P < 0.01) in cumulus-oocyte-complexes (COCs) during IVM progress. The proportion of arrested germinal vesicles (GV) increased in oocytes treated with EGF+GD1a (41.6 ± 1.5%) at the IVM I stage. Upon completion of meiotic maturation, the proportion of metaphase II (M II) was significantly higher (P < 0.05) in the EGF+GD1a (89.9 ± 3.6%) treated group. After IVF, the percentage of penetrated oocytes was significantly higher (P < 0.05) in the EGF+GD1a (89.1 ± 2.3%) treated group than in the control group. Furthermore, exogenous GD1a treatment improved the developmental competence and quality of blastocysts during preimplantation embryo development stage. These results suggest that ganglioside GD1a may play an important role in IVM mechanisms of porcine maturation capacity. Furthermore, our findings will be helpful for better promoting the embryo development and blastocyst quality in pigs.-
dc.publisherSociety Reproduction & Development-Srd-
dc.titleGanglioside GD1a promotes oocyte maturation, furthers preimplantation development, and increases blastocyst quality in pigs-
dc.title.alternativeGanglioside GD1a promotes oocyte maturation, furthers preimplantation development, and increases blastocyst quality in pigs-
dc.typeArticle-
dc.citation.titleJournal of Reproduction and Development-
dc.citation.number3-
dc.citation.endPage255-
dc.citation.startPage249-
dc.citation.volume62-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName김진우-
dc.contributor.alternativeName박효진-
dc.contributor.alternativeName채성규-
dc.contributor.alternativeName안재현-
dc.contributor.alternativeName도건엽-
dc.contributor.alternativeName추영국-
dc.contributor.alternativeName박정준-
dc.contributor.alternativeName정배동-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName구덕본-
dc.identifier.bibliographicCitationJournal of Reproduction and Development, vol. 62, no. 3, pp. 249-255-
dc.identifier.doi10.1262/jrd.2015-083-
dc.subject.keywordGanglioside GD1a-
dc.subject.keywordMeiotic maturation-
dc.subject.keywordPigs-
dc.subject.keywordPreimplantation development-
dc.subject.localGanglioside GD1a-
dc.subject.localMeiotic maturation-
dc.subject.localPigs-
dc.subject.localpigs-
dc.subject.localPig-
dc.subject.localpig-
dc.subject.localPreimplantation development-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.