Gluconic acid production by gad mutant of Klebsiella pneumoniae

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Title
Gluconic acid production by gad mutant of Klebsiella pneumoniae
Author(s)
D Wang; C Wang; D Wei; Z Shi; Chul Ho Kim; B Jiang; Z Han; J Hao
Bibliographic Citation
World Journal of Microbiology & Biotechnology, vol. 32, no. 8, pp. 132-132
Publication Year
2016
Abstract
Klebsiella pneumoniae produces many economically important chemicals. Using glucose as a carbon source, the main metabolic product in K. pneumoniae is 2,3-butanediol. Gluconic acid is an intermediate of the glucose oxidation pathway. In the current study, a metabolic engineering strategy was used to develop a gluconic acid-producing K. pneumoniae strain. Deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. Gluconic acid accumulation by K. pneumoniae Δgad was an acid-dependent aerobic process, with accumulation observed at pH 5.5 or lower, and at higher levels of oxygen supplementation. Under all other conditions tested, 2,3-butanediol was the main metabolic product of the process. In fed batch fermentation, a final concentration of 422?g/L gluconic acid was produced by K. pneumoniae Δgad, and the conversion ratio of glucose to gluconic acid reached 1?g/g. The K. pneumoniae Δgad described in this study is the first genetically modified strain used for gluconic acid production, and this optimized method for gluconic acid production may have important industrial applications. Graphical Gluconic acid is an intermediate of this glucose oxidation pathway. Deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. In fed batch fermentation, a final concentration of 422?g/L gluconic acid was produced by the K. pneumoniae Δgad strain, and the conversion ratio of glucose to gluconic acid reached 1?g/g.[Figure not available: see fulltext.]
Keyword
Gluconate dehydrogenaseGluconic acidGlucose oxidation pathwayKlebsiella pneumoniae
ISSN
0959-3993
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11274-016-2080-x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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