miR-6734 up-regulates p21 gene expression and induces cell cycle arrest and apoptosis in colon cancer cells

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dc.contributor.authorMoo Rim Kang-
dc.contributor.authorKi Hwan Park-
dc.contributor.authorJeong Ook Yang-
dc.contributor.authorChang Woo Lee-
dc.contributor.authorSoo Jin Oh-
dc.contributor.authorJi Eun Yun-
dc.contributor.authorMyeong Youl Lee-
dc.contributor.authorS B Han-
dc.contributor.authorJong Soon Kang-
dc.date.accessioned2017-04-19T10:25:59Z-
dc.date.available2017-04-19T10:25:59Z-
dc.date.issued2016-
dc.identifier.issn1932-6203-
dc.identifier.uri10.1371/journal.pone.0160961ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13385-
dc.description.abstractRecently, microRNAs have been implicated in the regulation of gene expression in terms of both gene silencing and gene activation. Here, we investigated the effects of miR-6734, which has a sequence homology with a specific region of p21WAF1/CIP1 (p21) promoter, on cancer cell growth and the mechanisms involved in this effect. miR-6734 up-regulated p21 expression at both mRNA and protein levels and chromatin immunoprecipitation analysis using biotin-labeled miR-6734 confirmed the association of miR-6734 with p21 promoter. Moreover, miR-6734 inhibited cancer cell growth and induced cell cycle arrest and apoptosis in HCT-116 cells, which was abolished by knockdown of p21. The phosphorylation of Rb and the cleavage of caspase 3 and PARP were suppressed by miR-6734 transfection in HCT-116 cells and these effects were also reversed by p21 knockdown. In addition, miR-6734 transfection caused prolonged induction of p21 gene and modification of histones in p21 promoter, which are typical aspects of a phenomenon referred to as RNA activation (RNAa). Collectively, our results demonstrated that miR-6734 inhibits the growth of colon cancer cells by up-regulating p21 gene expression and subsequent induction of cell cycle arrest and apoptosis, suggesting its role as an important endogenous regulator of cancer cell proliferation and survival.-
dc.publisherPublic Library of Science-
dc.titlemiR-6734 up-regulates p21 gene expression and induces cell cycle arrest and apoptosis in colon cancer cells-
dc.title.alternativemiR-6734 up-regulates p21 gene expression and induces cell cycle arrest and apoptosis in colon cancer cells-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number8-
dc.citation.endPagee0160961-
dc.citation.startPagee0160961-
dc.citation.volume11-
dc.contributor.affiliatedAuthorMoo Rim Kang-
dc.contributor.affiliatedAuthorKi Hwan Park-
dc.contributor.affiliatedAuthorJeong Ook Yang-
dc.contributor.affiliatedAuthorChang Woo Lee-
dc.contributor.affiliatedAuthorSoo Jin Oh-
dc.contributor.affiliatedAuthorJi Eun Yun-
dc.contributor.affiliatedAuthorMyeong Youl Lee-
dc.contributor.affiliatedAuthorJong Soon Kang-
dc.contributor.alternativeName강무림-
dc.contributor.alternativeName박기환-
dc.contributor.alternativeName양정욱-
dc.contributor.alternativeName이창우-
dc.contributor.alternativeName오수진-
dc.contributor.alternativeName윤지은-
dc.contributor.alternativeName이명열-
dc.contributor.alternativeName한상배-
dc.contributor.alternativeName강종순-
dc.identifier.bibliographicCitationPLoS One, vol. 11, no. 8, pp. e0160961-e0160961-
dc.identifier.doi10.1371/journal.pone.0160961-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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