DUSP1 induces paclitaxel resistance through the regulation of p-glycoprotein expression in human ovarian cancer cells

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dc.contributor.authorYu-Seon Kang-
dc.contributor.authorHyun-Jeong Seok-
dc.contributor.authorEun-Jeong Jeong-
dc.contributor.authorYuna Kim-
dc.contributor.authorS J Yun-
dc.contributor.authorJeong Ki Min-
dc.contributor.authorS J Kim-
dc.contributor.authorJang Seong Kim-
dc.date.accessioned2017-04-19T10:26:22Z-
dc.date.available2017-04-19T10:26:22Z-
dc.date.issued2016-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2016.07.035ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13404-
dc.description.abstractThe heterogeneity and genetic instability of ovarian cancer cells often lead to the development of drug resistance, closely related with the increased cancer-related mortality. In this study, we investigated the role of dual-specificity phosphatase 1 (DUSP1) in the development of the resistance in human ovarian cancer cells against paclitaxel. Overexpression of DUSP1 in HeyA8 human ovarian cancer cells (HeyA8-DUSP1) up-regulated the expression of the drug efflux pump, p-glycoprotein. Consequently, HeyA8-DUSP1 cells are highly resistant to paclitaxel, with the resistance comparable to that of a multi-drug resistance cell line (HeyA8-MDR). Moreover, over expression of DUSP1 significantly increased the activation of p38 MAPK, leaving the activation of ERK1/2 and JNK1/2 unaffected. Pharmacological suppression of p38 MAPK activity prevents the up-regulation of p-glycoprotein expression and the consequent resistance against paclitaxel in HeyA8-DUSP1 cells. By contrast, HeyA8-MDR cells expressed a significantly higher level of DUSP1, but treatment with small interference RNA against DUSP1 significantly suppressed the expression of p-glycoprotein and the resistance against paclitaxel in HeyA8-MDR cells. Ectopic expression of MKK3, an upstream activator of p38 MAPK, significantly up-regulated the expression of p-glycoprotein and increased the consequent resistance against paclitaxel in HeyA8 cells. Collectively, these data indicated that DUSP1 may induce the resistance against paclitaxel through the p38 MAPK-mediated overexpression of p-glycoprotein in human ovarian cancer cells.-
dc.publisherElsevier-
dc.titleDUSP1 induces paclitaxel resistance through the regulation of p-glycoprotein expression in human ovarian cancer cells-
dc.title.alternativeDUSP1 induces paclitaxel resistance through the regulation of p-glycoprotein expression in human ovarian cancer cells-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number0-
dc.citation.endPage409-
dc.citation.startPage403-
dc.citation.volume478-
dc.contributor.affiliatedAuthorYu-Seon Kang-
dc.contributor.affiliatedAuthorHyun-Jeong Seok-
dc.contributor.affiliatedAuthorEun-Jeong Jeong-
dc.contributor.affiliatedAuthorYuna Kim-
dc.contributor.affiliatedAuthorJeong Ki Min-
dc.contributor.affiliatedAuthorJang Seong Kim-
dc.contributor.alternativeName강유선-
dc.contributor.alternativeName석현정-
dc.contributor.alternativeName정은정-
dc.contributor.alternativeName김윤아-
dc.contributor.alternativeName윤석중-
dc.contributor.alternativeName민정기-
dc.contributor.alternativeName김선진-
dc.contributor.alternativeName김장성-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 478, pp. 403-409-
dc.identifier.doi10.1016/j.bbrc.2016.07.035-
dc.subject.keywordDrug resistance-
dc.subject.keywordDUSP1-
dc.subject.keywordOvarian carcinoma-
dc.subject.keywordP-glycoprotein-
dc.subject.keywordPaclitaxel-
dc.subject.localDrug resistance-
dc.subject.localDrug-resistance-
dc.subject.localdrug-resistance-
dc.subject.localdrug resistance-
dc.subject.localDUSP1-
dc.subject.localOvarian carcinoma-
dc.subject.localP-glycoprotein-
dc.subject.localPaclitaxel-
dc.subject.localpaclitaxel-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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