DC Field | Value | Language |
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dc.contributor.author | Yu-Seon Kang | - |
dc.contributor.author | Hyun-Jeong Seok | - |
dc.contributor.author | Eun-Jeong Jeong | - |
dc.contributor.author | Yuna Kim | - |
dc.contributor.author | S J Yun | - |
dc.contributor.author | Jeong Ki Min | - |
dc.contributor.author | S J Kim | - |
dc.contributor.author | Jang Seong Kim | - |
dc.date.accessioned | 2017-04-19T10:26:22Z | - |
dc.date.available | 2017-04-19T10:26:22Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | 10.1016/j.bbrc.2016.07.035 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/13404 | - |
dc.description.abstract | The heterogeneity and genetic instability of ovarian cancer cells often lead to the development of drug resistance, closely related with the increased cancer-related mortality. In this study, we investigated the role of dual-specificity phosphatase 1 (DUSP1) in the development of the resistance in human ovarian cancer cells against paclitaxel. Overexpression of DUSP1 in HeyA8 human ovarian cancer cells (HeyA8-DUSP1) up-regulated the expression of the drug efflux pump, p-glycoprotein. Consequently, HeyA8-DUSP1 cells are highly resistant to paclitaxel, with the resistance comparable to that of a multi-drug resistance cell line (HeyA8-MDR). Moreover, over expression of DUSP1 significantly increased the activation of p38 MAPK, leaving the activation of ERK1/2 and JNK1/2 unaffected. Pharmacological suppression of p38 MAPK activity prevents the up-regulation of p-glycoprotein expression and the consequent resistance against paclitaxel in HeyA8-DUSP1 cells. By contrast, HeyA8-MDR cells expressed a significantly higher level of DUSP1, but treatment with small interference RNA against DUSP1 significantly suppressed the expression of p-glycoprotein and the resistance against paclitaxel in HeyA8-MDR cells. Ectopic expression of MKK3, an upstream activator of p38 MAPK, significantly up-regulated the expression of p-glycoprotein and increased the consequent resistance against paclitaxel in HeyA8 cells. Collectively, these data indicated that DUSP1 may induce the resistance against paclitaxel through the p38 MAPK-mediated overexpression of p-glycoprotein in human ovarian cancer cells. | - |
dc.publisher | Elsevier | - |
dc.title | DUSP1 induces paclitaxel resistance through the regulation of p-glycoprotein expression in human ovarian cancer cells | - |
dc.title.alternative | DUSP1 induces paclitaxel resistance through the regulation of p-glycoprotein expression in human ovarian cancer cells | - |
dc.type | Article | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 409 | - |
dc.citation.startPage | 403 | - |
dc.citation.volume | 478 | - |
dc.contributor.affiliatedAuthor | Yu-Seon Kang | - |
dc.contributor.affiliatedAuthor | Hyun-Jeong Seok | - |
dc.contributor.affiliatedAuthor | Eun-Jeong Jeong | - |
dc.contributor.affiliatedAuthor | Yuna Kim | - |
dc.contributor.affiliatedAuthor | Jeong Ki Min | - |
dc.contributor.affiliatedAuthor | Jang Seong Kim | - |
dc.contributor.alternativeName | 강유선 | - |
dc.contributor.alternativeName | 석현정 | - |
dc.contributor.alternativeName | 정은정 | - |
dc.contributor.alternativeName | 김윤아 | - |
dc.contributor.alternativeName | 윤석중 | - |
dc.contributor.alternativeName | 민정기 | - |
dc.contributor.alternativeName | 김선진 | - |
dc.contributor.alternativeName | 김장성 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, vol. 478, pp. 403-409 | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.07.035 | - |
dc.subject.keyword | Drug resistance | - |
dc.subject.keyword | DUSP1 | - |
dc.subject.keyword | Ovarian carcinoma | - |
dc.subject.keyword | P-glycoprotein | - |
dc.subject.keyword | Paclitaxel | - |
dc.subject.local | Drug resistance | - |
dc.subject.local | Drug-resistance | - |
dc.subject.local | drug-resistance | - |
dc.subject.local | drug resistance | - |
dc.subject.local | DUSP1 | - |
dc.subject.local | Ovarian carcinoma | - |
dc.subject.local | P-glycoprotein | - |
dc.subject.local | Paclitaxel | - |
dc.subject.local | paclitaxel | - |
dc.description.journalClass | Y | - |
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