Antibody-mediated rejection in sensitized nonhuman primates: modeling human biology

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Title
Antibody-mediated rejection in sensitized nonhuman primates: modeling human biology
Author(s)
C K Burghuber; J Kwun; E J Page; M Manook; A C Gibby; F V Leopardi; M Song; A B Farris III; Jung Joo Hong; F Villinger; A B Adams; N N Iwakoshi; S J Knechtle
Bibliographic Citation
American Journal of Transplantation, vol. 16, no. 6, pp. 1726-1738
Publication Year
2016
Abstract
We have established a model of sensitization in nonhuman primates and tested two immunosuppressive regimens. Animals underwent fully mismatched skin transplantation, and donor-specific antibody (DSA) response was monitored by flow cross-match. Sensitized animals subsequently underwent kidney transplantation from their skin donor. Immunosuppression included tacrolimus, mycophenolate, and methylprednisolone. Three animals received basiliximab induction; compared with nonsensitized animals, they showed a shorter mean survival time (4.7 ± 3.1 vs. 187 ± 88 days). Six animals were treated with T cell depletion (anti-CD4/CD8 mAbs), which prolonged survival (mean survival time 21.6 ± 19.0 days). All presensitized animals showed antibody-mediated rejection (AMR). In two of three basiliximab-injected animals, cellular rejection (ACR) was prominent. After T cell depletion, three of six monkeys experienced early acute rejection within 8 days with histological evidence of thrombotic microangiopathy and AMR. The remaining three monkeys survived 27-44 days, with mixed AMR and ACR. Most T cell-depleted animals experienced a rebound of DSA that correlated with deteriorating kidney function. We also found an increase in proliferating memory B cells (CD20+CD27+IgD-Ki67+), lymph node follicular helper T cells (ICOS+PD-1hiCXCR5+CD4+), and germinal center (GC) response. Depletion controlled cell-mediated rejection in sensitized nonhuman primates better than basiliximab, yet grafts were rejected with concomitant DSA rise. This model provides an opportunity to test novel desensitization strategies.
ISSN
1600-6135
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/ajt.13688
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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