Microcystin biosynthesis and mcyA expression in geographically distinct Microcystis strains under different nitrogen, phosphorus and boron regimes

Cited 15 time in scopus
Metadata Downloads
Title
Microcystin biosynthesis and mcyA expression in geographically distinct Microcystis strains under different nitrogen, phosphorus and boron regimes
Author(s)
A Srivastava; So Ra KoChi-Yong AhnHee-Mock Oh; A K Ravi; R K Asthana
Bibliographic Citation
Biomed Research International, vol. 2016, pp. 5985987-5985987
Publication Year
2016
Abstract
Roles of nutrients and other environmental variables in development of cyanobacterial bloom and its toxicity are complex and not well understood. We have monitored the photoautotrophic growth, total microcystin concentration, and microcystins synthetase gene (mcyA) expression in lab-grown strains of Microcystis NIES 843 (reference strain), KW (Wangsong Reservoir, South Korea), and Durgakund (Varanasi, India) under different nutrient regimes (nitrogen, phosphorus, and boron). Higher level of nitrogen and boron resulted in increased growth (avg. 5 and 6.5 Chl a mg/L, resp.), total microcystin concentrations (avg. 1.185 and 7.153 mg/L, resp.), and mcyA transcript but its expression was not directly correlated with total microcystin concentrations in the target strains. Interestingly, Durgakund strain had much lower microcystin content and lacked microcystin-YR variant over NIES 843 and KW. It is inferred that microcystin concentration and its variants are strain specific. We have also examined the heterotrophic bacteria associated with cyanobacterial bloom in Durgakund Pond and Wangsong Reservoir which were found to be enriched in Alpha-, Beta-, and Gammaproteobacteria and that could influence the bloom dynamics.
ISSN
2314-6133
Publisher
Hindawi Ltd
DOI
http://dx.doi.org/10.1155/2016/5985987
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.