Highly sensitive and rapid fluorescence detection with a portable FRET analyzer

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dc.contributor.authorHaseong Kim-
dc.contributor.authorGui Hwan Han-
dc.contributor.authorY Fu-
dc.contributor.authorJ Gam-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2017-04-19T10:27:24Z-
dc.date.available2017-04-19T10:27:24Z-
dc.date.issued2016-
dc.identifier.issn1940087X-
dc.identifier.uri10.3791/54144ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13437-
dc.description.abstractRecent improvements in Forster resonance energy transfer (FRET) sensors have enabled their use to detect various small molecules including ions and amino acids. However, the innate weak signal intensity of FRET sensors is a major challenge that prevents their application in various fields and makes the use of expensive, high-end fluorometers necessary. Previously, we built a cost-effective, high-performance FRET analyzer that can specifically measure the ratio of two emission wavelength bands (530 and 480 nm) to achieve high detection sensitivity. More recently, it was discovered that FRET sensors with bacterial periplasmic binding proteins detect ligands with maximum sensitivity in the critical temperature range of 50-55 °C. This report describes a protocol for assessing sugar content in commercially-available beverage samples using our portable FRET analyzer with a temperature-specific FRET sensor. Our results showed that the additional preheating process of the FRET sensor significantly increases the FRET ratio signal, to enable more accurate measurement of sugar content. The custom-made FRET analyzer and sensor were successfully applied to quantify the sugar content in three types of commercial beverages. We anticipate that further size reduction and performance enhancement of the equipment will facilitate the use of hand-held analyzers in environments where high-end equipment is not available.-
dc.publisherJournal of Visualized Experiments-
dc.titleHighly sensitive and rapid fluorescence detection with a portable FRET analyzer-
dc.title.alternativeHighly sensitive and rapid fluorescence detection with a portable FRET analyzer-
dc.typeArticle-
dc.citation.titleJournal of Visualized Experiments-
dc.citation.number116-
dc.citation.endPagee54144-
dc.citation.startPagee54144-
dc.citation.volume2016-
dc.contributor.affiliatedAuthorHaseong Kim-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName김하성-
dc.contributor.alternativeName한귀환-
dc.contributor.alternativeNameFu-
dc.contributor.alternativeName감종식-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationJournal of Visualized Experiments, vol. 2016, no. 116, pp. e54144-e54144-
dc.identifier.doi10.3791/54144-
dc.subject.keywordBiochemistry-
dc.subject.keywordFluorescence resonance energy transfer-
dc.subject.keywordFluorometer-
dc.subject.keywordFood assessment-
dc.subject.keywordForster resonance energy transfer (FRET)-
dc.subject.keywordIssue 116-
dc.subject.keywordPoint-of-care testing-
dc.subject.keywordPortable device-
dc.subject.keywordSugar content-
dc.subject.localBiochemistry-
dc.subject.localFluorescence resonance energy transfer-
dc.subject.localFluorescence resonance energy transfer (FRET)-
dc.subject.localFluorometer-
dc.subject.localFood assessment-
dc.subject.localForster resonance energy transfer (FRET)-
dc.subject.localIssue 116-
dc.subject.localPoint-of-care testing-
dc.subject.localPoint-of-care testing (POCT)-
dc.subject.localPortable device-
dc.subject.localSugar content-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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