Direct protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins

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dc.contributor.authorS Kim-
dc.contributor.authorW Ko-
dc.contributor.authorBong Hyun Sung-
dc.contributor.authorS C Kim-
dc.contributor.authorH S Lee-
dc.date.accessioned2017-04-19T10:28:28Z-
dc.date.available2017-04-19T10:28:28Z-
dc.date.issued2016-
dc.identifier.issn0968-0896-
dc.identifier.uri10.1016/j.bmc.2016.09.035ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13481-
dc.description.abstractProteins often function as complex structures in conjunction with other proteins. Because these complex structures are essential for sophisticated functions, developing protein?protein conjugates has gained research interest. In this study, site-specific protein?protein conjugation was performed by genetically incorporating an azide-containing amino acid into one protein and a bicyclononyne (BCN)-containing amino acid into the other. Three to four sites in each of the proteins were tested for conjugation efficiency, and three combinations showed excellent conjugation efficiency. The genetic incorporation of unnatural amino acids (UAAs) is technically simple and produces the mutant protein in high yield. In addition, the conjugation reaction can be conducted by simple mixing, and does not require additional reagents or linker molecules. Therefore, this method may prove very useful for generating protein?protein conjugates and protein complexes of biochemical significance-
dc.publisherElsevier-
dc.titleDirect protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins-
dc.title.alternativeDirect protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins-
dc.typeArticle-
dc.citation.titleBioorganic & Medicinal Chemistry-
dc.citation.number22-
dc.citation.endPage5822-
dc.citation.startPage5816-
dc.citation.volume24-
dc.contributor.affiliatedAuthorBong Hyun Sung-
dc.contributor.alternativeName김상길-
dc.contributor.alternativeName고우석-
dc.contributor.alternativeName성봉현-
dc.contributor.alternativeName김선창-
dc.contributor.alternativeName이현수-
dc.identifier.bibliographicCitationBioorganic & Medicinal Chemistry, vol. 24, no. 22, pp. 5816-5822-
dc.identifier.doi10.1016/j.bmc.2016.09.035-
dc.subject.keywordClick reaction-
dc.subject.keywordGenetic incorporation-
dc.subject.keywordProteinprotein conjugation-
dc.subject.keywordUnnatural amino acids-
dc.subject.localClick reaction-
dc.subject.localGenetic incorporation-
dc.subject.localProteinprotein conjugation-
dc.subject.localUnnatural amino acids-
dc.subject.localunnatural amino acid-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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