Cited 3 time in
- Title
- Plasma-treated flexible aminoclay-decorated electrospun nanofibers for neural stem cell self-renewal
- Author(s)
- Y C Lee; H U Lee; Minhyung Lee; Janghwan Kim; Y S Huh
- Bibliographic Citation
- Journal of Nanoscience and Nanotechnology, vol. 16, pp. 1392-1395
- Publication Year
- 2016
- Abstract
- Biocompatible Mg- And Fe-aminoclays (MgAC and FeAC)-decorated polyacrylonitrile (PAN) nanofibers (NFs, diameter range: 190-380 nm) are prepared by the electrospinning process. There is a large increase in the biomolecular activities of the PAN NFs that were oxygen plasma (OP)-treated (the OPNFs) relative to those of the pristine PAN NFs, due to the OP treatment's carboxylation and/or hydroxylation of the PAN NF surfaces. With morphological observation by scanning electron microscopy (SEM), and following further confirmation of the Fourier-transform infrared (FI-IR) spectra of the as-prepared AC-OPNFs, human neural stem cell (NSC) self-renewal is tested, focusing on the relevant discrepancies among the AC-OPNFs, OPNFs, and pristine PAN NFs as flexible cellular matrices. Interestingly, NSCs are attached well on four NFs without conventional coating materials. Self-renewal of NSCs is confirmed by marker expressions such as PAX6 and N-CADHERIN. Among four NFs, FeAC-OPNFs shows the best property of NSC self-renewal. It is expected that AC-OPNFs can be xeno-free and protein-free extracellular matrices for supporting human NSC self-renewal.
- Keyword
- AminoclayElectrospun fiberNeural stem cellSelf-renewal
- ISSN
- 1533-4880
- Publisher
- Amer Scientific Publishers
- DOI
- http://dx.doi.org/10.1166/jnn.2016.11911
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
- Files in This Item:
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