Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

Cited 8 time in scopus
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Title
Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration
Author(s)
H Kim; D H Kang; K H Koo; S Lee; S M Kim; Janghwan Kim; M H Yoon; S Y Kim; E G Yang
Bibliographic Citation
Nanoscale, vol. 8, no. 42, pp. 18087-18097
Publication Year
2016
Abstract
The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.
ISSN
2040-3364
Publisher
Royal Soc Chem
DOI
http://dx.doi.org/10.1039/c6nr06203b
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
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