The OsCYP19-4 gene is expressed as multiple alternatively spliced transcripts encoding isoforms with distinct cellular localizations and PPIase activities under cold stress
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- The OsCYP19-4 gene is expressed as multiple alternatively spliced transcripts encoding isoforms with distinct cellular localizations and PPIase activities under cold stress
- Areum Lee; Sang Sook Lee; Won Yong Jung; Hyun Ji Park; Bo Ra Lim; Hyun Soon Kim; J C Ahn; Hye Sun Cho
- Bibliographic Citation
- International Journal of Molecular Sciences, vol. 17, pp. 1154-1154
- Publication Year
- Alternative splicing (AS) is an important molecular mechanism by which single genes can generate multiple mRNA isoforms. We reported previously that, in Oryza sativa, the cyclophilin 19-4 (OsCYP19-4.1) transcript was significantly upregulated in response to cold stress, and that transgenic plants were cold tolerant. Here we show that, under cold stress, OsCYP19-4 produces eight transcript variants by intron retention and exon skipping, resulting in production of four distinct protein isoforms. The OsCYP19-4 AS isoforms exhibited different cellular localizations in the epidermal cells: in contrast to OsCYP19-4.1, the OsCYP19-4.2 and OsCYP19-4.3 proteins were primarily targeted to guard and subsidiary cells, whereas OsCYP19-4.5, which consists largely of an endoplasmic reticulum (ER) targeting signal, was co-localized with the RFP-BiP marker in the ER. In OsCYP19-4.2, the key residues of the PPIase domain are altered; consistent with this, recombinant OsCYP19-4.2 had significantly lower PPIase activity than OsCYP19-4.1 in vitro. Specific protein-protein interactions between OsCYP19-4.2/3 and AtRCN1 were verified in yeast two-hybrid (Y2H) and bimolecular fluoresence complementation (BiFC assays), although the OsCYP19-4 isoforms could not bind each other. Based on these results, we propose that two OsCYP19-4 AS isoforms, OsCYP19-4.2 and OsCYP19-4.3, play roles linking auxin transport and cold stress via interactions with RCN1.
- Alternative splicingCold stressIsoformOsCYP19-4PPIase activityRCN1 interactor
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- Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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