Loktanella marina sp. nov., isolated from seawater

Abstract

A Gram-stain-negative, coccoid- or short-rod-shaped and non-motile bacterial strain, designated MDM-7T, was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain MDM 7T grew optimally at pH 7.08.0, at 3°C and in the presence of 2 3% (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-7T fell within the clade comprising species of the genus Loktanella, clustering with the type strains of Loktanella vestfoldensisand Loktanella agnita, with which it exhibited 96.1 and 95.6% similarity, respectively. 16S rRNA gene sequence similarity values between strain MDM-7T and the type strains of other Loktanella species were 93.1 95.0 %. The DNA G+C content of strain MDM-7T was 62.6 mol%. Strain MDM-7T contained Q 10 as the predominant ubiquinone and summed feature 8 (C18: 1ω7c and/ or C18: 1ω6c) as the major fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain MDM 7T is distinguishable from recognized Loktanella species. On the basis of the data presented, strain MDM-7T is considered to represent a novel species of the genus Loktanella, for which the name Loktanella marina sp. nov. is proposed. The type strain is MDM-7T KCTC 42722T CECT 8899T.