Proteomic assessment of the relevant factors affecting pork meat quality associated with Longissimus dorsi Muscles in Duroc pigs

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Title
Proteomic assessment of the relevant factors affecting pork meat quality associated with Longissimus dorsi Muscles in Duroc pigs
Author(s)
J H Cho; R H Lee; Young Joo Jeon; S M Park; J C Shin; Seok Ho Kim; J Y Jeong; H S Kang; N J Choi; K S Seo; Y S Cho; M S Kim; S Ko; J M Seo; S Y Lee; J H Shim; J I Chae
Bibliographic Citation
Asian-Australasian Journal of Animal Sciences, vol. 29, no. 11, pp. 1653-1663
Publication Year
2016
Abstract
Meat quality is a complex trait influenced by many factors, including genetics, nutrition, feeding environment, animal handling, and their interactions. To elucidate relevant factors affecting pork quality associated with oxidative stress and muscle development, we analyzed protein expression in high quality longissimus dorsi muscles (HQLD) and low quality longissimus dorsi muscles (LQLD) from Duroc pigs by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis. Between HQLD (n = 20) and LQLD (n = 20) Duroc pigs, 24 differentially expressed proteins were identified by LC-MS/MS. A total of 10 and 14 proteins were highly expressed in HQLD and LQLD, respectively. The 24 proteins have putative functions in the following seven categories: catalytic activity (31%), ATPase activity (19%), oxidoreductase activity (13%), cytoskeletal protein binding (13%), actin binding (12%), calcium ion binding (6%), and structural constituent of muscle (6%). Silver-stained image analysis revealed significant differential expression of lactate dehydrogenase A (LDHA) between HQLD and LQLD Duroc pigs. LDHA was subjected to in vitro study of myogenesis under oxidative stress conditions and LDH activity assay to verification its role in oxidative stress. No significant difference of mRNA expression level of LDHA was found between normal and oxidative stress condition. However, LDH activity was significantly higher under oxidative stress condition than at normal condition using in vitro model of myogenesis. The highly expressed LDHA was positively correlated with LQLD. Moreover, LDHA activity increased by oxidative stress was reduced by antioxidant resveratrol. This paper emphasizes the importance of differential expression patterns of proteins and their interaction for the development of meat quality traits. Our proteome data provides valuable information on important factors which might aid in the regulation of muscle development and the improvement of meat quality in longissimus dorsi muscles of Duroc pigs under oxidative stress conditions.
Keyword
DurocLiquid Chromatography-tandem Mass SpectrometryLongissimus dorsi MuscleMeat QualityOxidative Stress
ISSN
1011-2367
Publisher
Asian-Australasian Assoc Animal Production Soc
DOI
http://dx.doi.org/10.5713/ajas.16.0050
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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