Homeobox A9 directly targeted by miR-196b regulates aggressiveness through nuclear factor-kappa B activity in non-small cell lung cancer cells = miR-196b의 표적유전자 homeoboxA9는 비소세포 폐암에서 NF-KB 활성을 통해서 악성 조절

Cited 32 time in scopus
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Title
Homeobox A9 directly targeted by miR-196b regulates aggressiveness through nuclear factor-kappa B activity in non-small cell lung cancer cells = miR-196b의 표적유전자 homeoboxA9는 비소세포 폐암에서 NF-KB 활성을 통해서 악성 조절
Author(s)
S L Yu; Dong Chul Lee; H A Sohn; S Y Lee; H S Jeon; J H Lee; C G Park; H Y Lee; Young Il Yeom; J W Son; Y S Yoon; J Kang
Bibliographic Citation
Molecular Carcinogenesis, vol. 55, no. 12, pp. 1915-1926
Publication Year
2016
Abstract
MicroRNAs (miRNAs) are recognized as crucial posttranscriptional regulators of gene expression, and play critical roles as oncogenes or tumor suppressors in various cancers. Here, we show that miR-196b is upregulated in mesenchymal-like-state non-small cell lung cancer (NSCLC) cells and lung cancer tissues. Moreover, miR-196b upregulation stimulates cell invasion and a change in cell morphology to a spindle shape via loss of cell-to-cell contacts. We identified homeobox A9 (HOXA9) as a target gene of miR-196b by using public databases such as TargetScan, miRDB, and microRNA.org. HOXA9 expression is inversely correlated with miR-196b levels in clinical NSCLC samples as compared to that in corresponding control samples, and with the migration and invasion of NSCLC cells. Ectopic expression of HOXA9 resulted in a suppression of miR-196b-induced cell invasion, and HOXA9 reexpression increased E-cadherin expression. Furthermore, HOXA9 potently attenuated the expression of snail family zinc finger 2 (SNAI2/SLUG) and matrix metallopeptidase 9 (MMP9) by controlling the binding of nuclear factor?kappa B to the promoter of SLUG and MMP9 genes, respectively. Therefore, we suggest that HOXA9 plays a central role in controlling the aggressive behavior of lung cancer cells and that miR-196b can serve as a potential target for developing anticancer agents.
Keyword
EMTHOXA9miR-196bNF-κBNSCLC
ISSN
0899-1987
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/mc.22439
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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